Echinops phaeocephalus (Asteraceae) Türünün Antimikrobiyal ve Antioksidan Aktivitesi

Bu çalışmada, E. phaeocephalus türünün antimikrobiyal ve antioksidan aktivitesini araştırmak amaçlanmıştır. E. phaeocephalus türünün ekstraksiyonları hekzan, methanol ve kloroform olmak üzere üç farklı çözücü kullanılarak elde edilmiştir. Antioksidan kapasitesi DPPH radikali süpürücü aktivite tayini, toplam fenolik içeriğin belirlenmesi ve toplam flavanoid içeriğinin belirlenmesi testleri yapılarak belirlenmiştir. Antimikrobiyal aktiviteleri disk difüzyon testi ve mikrodilüsyon (MIC) yöntemi kullanılarak araştırılmıştır. Çözücüler ekstraksiyon verimi bakımından değerlendirildiğinde metanolün diğer çözücülerden daha etkili olduğu tespit edilmiştir. Echinops phaeocephalus türü ekstraktlarının mikroorganizmalar üzerinde değişen etkilere sahip olduğu gözlenmiştir. Antioksidan aktivite tayini testleri neticesinde, fenolik madde miktarı en yüksek hekzan ile elde edilen ekstraktda gözlenirken, flavanoid madde içeriği bakımından zengin olan ekstrakt metanol ile elde edilen ekstrakt olmuştur. DPPH radikali süpürücü aktivite tayini sonuçlarında ise özellikle metanol ve hekzan ekstraktlarından yüksek verim elde edilmiştir. Elde edilen sonuçlar neticesinde de E. phaeocephalus türünün önemli derecede antioksidan ve antimikrobiyal aktiviteye sahip olduğu belirlenmiştir.

Anahtar Kelimeler:

fenolik içerik, Compositae, flavanoid, antibakterial, antifungal

Antimicrobial and Antioxidant Activity of Echinops phaeocephalus (Asteraceae)

The aim of this study was to investigate antimicrobial and antioxidant activity of Echinops phaeocephalus extracts prepared with different solvents. For antioxidant potential evaluation, extraction of E. phaeocephalus was obtained by three solvents of different polarities including hexane, methanol and chloroform. Antioxidant potential was evaluated by estimating DPPH radical scavenging activity, total phenolic activity and total flavonoid activity assay. Antimicrobial activity was determined by minimal inhibitory concentration (MIC) and disc diffusion tests. Efficiency of different solvents was compared for the yield of antioxidant extracts from samples. The study results indicated that, E. phaeocephalus could be an important natural antioxidant. The evaluations of solvents for effect of extraction resulted that methanol was more effective than other solvents. Echinops phaeocephalus derived extracts were observed to have varies effects on microorganisms. The results of the antioxidant activity assay indicated that the highest phenolic content was in the extract of hexane, while the highest flavonoid content was in methanol extract. Especially methanol and hexane extracts showed high efficiency according to DPPH radical scavenging activity results. Our results concluded that E. phaeocephalus sustains an important antioxidant and antimicrobial activity.

Keywords:

Compositae, phenolic content, flavonoid, antibacterial, antifungal,

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