Hüban GÖÇMEN, Selim ALÇAY, Ruben S. ROSALES, Anne RIDLEY

Teke ve Koçların Prepusyal Svap Örneklerinden Genital Mikoplazma Türlerinin Karakterizasyonu

Mikoplazma türleri küçük ruminantlarda mastitis, artritis, pnömoni ve reprodüktif bozukluklara neden olabilmektedir. Erkek hayvanların reprodüktif sistemlerinde bulunan mikoplazmalar; orşitis, balanopostitis ve anormal spermatozoa aktivitesi gibi hastalıklarla ilişkilendirilmiştir. Bu çalışmada, teke ve koçlarda reprodüktif infeksiyonlara neden olan mikoplazma türlerinin tespiti ve karakterizasyonu amaçlandı. Uludağ Üniversitesi Suni Tohumlama Merkezi’nde bulunan Saanen ırkı tekelerden ve Kıvırcık ırkı koçlardan toplamda 27 adet prepusyal svap örnekleri toplandı. Mikoplazma türlerinin izolasyon ve identifikasyonu için bakteriyolojik kültür yöntemleri, Polimeraz Zincir Reaksiyonu (PCR) ve Denatüre Edici Gradient Jel Elektroforezi (DGGE) metodu uygulandı. PCR-DGGE metodu ile orşitis semptomu gösteren koçların birinden M. bovigenitalium ve diğerinden M. arginini identifiye edilirken; semptom göstermeyen bir tekeden ise M. bovigenitalium identifiye edildi. Elde edilen sonuçlar ile Brusellozis negatif olan koç ve tekelerin testislerinde, fertilite azalmasına yol açan orşitise neden olan Mikoplazma türlerinin varlığı ortaya konmuştur.

Characterisation of Genital Mycoplasma Species from Preputial Swabs of Bucks and Rams

Mycoplasma species are a major cause of mastitis, arthritis, pneumonia and reproductive disorders in small ruminants. Mycoplasmas in reproductive systems of males have been associated with diseases as orchitis, balanoposthitis and abnormal spermatozoa activity. The aim of this study was to detect Mycoplasma species that cause reproductive infections in bucks and rams. Total of 27 preputial swabs was collected from Saanen bucks and Kıvırcık rams at the Artificial Insemination (AI) Center of Uludag University in Turkey. Bacteriological culture methods, followed by Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) were used to detect and identify Mycoplasma species. The PCR-DGGE method identified one M. bovigenitalium and one M. arginini from two cases of orchitis in rams, and another M. bovigenitalium was identified from a buck with no clinical signs. The results showed that Mycoplasma species were present in the testicles of rams and bucks that were negative for Brucellosis, and likely causative organisms of orchitis which lead to reduced fertility.

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