Submerged Kültürü ile Bacillus subtilis RSKK96'dan ?-Amilaz Üretimi

Bakteriyel tür Bacillus subtilis RSKK96'nın ekstrasellüler ?-amilaz ürettiği gözlenmiştir. Enzim sentezi 72. saatte optimum 37°C'de elde edilmiştir. Çeşitli karbon, azot ve aminoasit kaynakları ve kimyasalların ?-amilaz üretimi üzerine etkileri incelenmiştir. FeSO4, ZnSO4ve CuSO4 bakteriyel büyümeyi ve dolayısıyla ?-amilaz üretmini inhibe etmiştir. Maksimum ?-amilaz üretimi (858.6±41.9 U/mg) %0.5 pamuk sapı bulunan ortamda 72. saatte elde edilmiştir.

Alpha-Amylase Production by Bacillus subtilis RSKK96 in Submerged Cultivation

The bacterial strain Bacillus subtilis RSKK96 was shown to produce extracellular ?-amylase. Enzyme synthesis occurred at 72 h with an optimum of 37°C. Eff ects of various carbon, nitrogen, amino acid sources and chemicals on ?-amylase production were examined. FeSO4, ZnSO4 and CuSO4 inhibited bacterial growth as a result, amylase production. Maximum ?-amylase production (858.6±41.9 U/mg) was obtained in a medium containing 0.5% cotton stalk in 72 h.

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