Jalal SHAYEGH, Abolfazl BARZEGARI, Peyman MIKAILI

Molecular characteristics of Staphylococcus aureus isolates from buffaloes milk

Bu çalışmanın amacı, İranın Kuzey Batısında yerli mandaların sütlerinden izole edilen Staphylococcus aureus izolatlarında spa genlerinin aroA ve X bölgelerini analiz etmektir. Bu amaçla, yetmiş beş S. aureus izolatı PCR-RFLP yöntemi ile incelendi. AroA gen amplifikasyonu, yaklaşık olarak 1,153 bp büyüklüğünde olan tek bir amplikon oluşturmasına rağmen bu amplikon restriksiyon profil analizi ile genotip A (%9.3), B (%88) ve N (%2.7) olmak üzere üç farklı genotipi oluşturduğu belirlendi. spa geninin X bölgesinin amplifikasyonu 19, 15, 12 ve 29 izolatta sırasıyla 1110, 1130, 1160 ve 1190 bp büyüklüğünde fragman ürettiği gözlemlendi. Bu amplikonun HhaI enzimi ile kesimi, aynı PCR bant büyüklüğüne sahip izolatların aynı PCR-RFLP profillerinde olduğunu ve aynı PCR amplikonuna ait izolatlar içinde varyasyon olmadığını gösterdi.

Manda sütünden izole edilen Staphylococcus aureus türlerinin moleküler karakterizasyonu

The aim of the present study was to analyse the aroA and X region of spa genes on Staphylococcus aureus isolates collected from milks of native buffaloes in north-west of Iran. For this purpose, seventy five S. aureus isolates were examined by PCR-RFLP. Amplification of AroA gene revealed a single amplicon with a size of approximately 1,153 bp, but restriction profile analysis of this amplicon yielded three different genotypes including genotype A (9.3%), B (88%) and N (2.7%). Amplification of X region of the spa gene showed an amplicon of 1110; 1130; 1160; 1190 bp in 19, 15, 12 and 29 isolates, respectively. HhaI digestion of this amplicons demonstrated that the isolates having the same PCR band size were in the same PCR-RFLP patterns and there was not variation into isolates belonged to the same PCR amplicon.

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