Immunostimulating effect of levamisole on spleen and head-kidney leucocytes of rainbow trout (Oncorhynchus mykiss, walbaum 1792)

Bu çalışmada; levamisolün farklı dozlarının gökkuşağı alabalıklarının (Oncorhynchus mykiss) dalak ve ön böbrek lökositlerine etkisi in vitro olarak araştırılmıştır. Bu amaçla, levamisolün 10 μg ml 1 , 50 μg ml -1 ve 100 μg ml -1 dozunun uygulandığı bir kültür ortamında 15ºC’de 10 günlük dalak ve ön böbrek hücre kültürleri yapıldı. Lökosit düzeyi, glass-adherent NBT pozitif hücre aktivasyonu ve fagositik aktivite gibi nötrofillerin metabolitik aktivitelerinin ölçülmesi ile nonspesifik savunmadaki değişim ortaya konulmuştur. 10 μg ml -1 ve 50 μg ml -1 levamisol dozlarının uygulandığı deneme gruplarında bir artış gözlendiği (P0.05).

Anahtar Kelimeler:

bağışıklık sistemi, antihelmintikler, dalak, gökkuşağı alabalığı, levamizol, böbrekler, bağışıklık uyarımı, immunostimulanlar, bağışıklık, balık

Levamisolün gökkuşağı alabalığının (Oncorhynchus mykiss, walbaum 1792) dalak ve ön böbrek lökositlerine immunostimulatik etkisinin araştırılması

An in vitro cultivation method for spleen and head kidney organ section were used to investigate the ef ects of levamisole on the immune response. After 10 days of culture with 10 μg/ml, 50 μg/ml and 100 μg/ml or no levamisole in the culture media, the non specific defense reaction were measured by glass-adherent NBT (nitroblue tetrazolium) positive cells activation and phagocytic activity. Elevation in phagocytic activity was found for 10 μg/ml and 50 μg/ml levels of levamisole (P<0.001), but no dif erences were found at levels of phagocytic activity in between 100 μg/ml levamisole groups and control groups (P>0.05). In the spleen and head kidney leucocytes, increased response to levamisole was shawn for all doses in the glass-adherent NBT positive cells activation.

Keywords:

immune system, anthelmintics, spleen, rainbow trout, levamisole, kidneys, immunostimulation, immunostimulants, immunity, fishes,

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1. Seljelid R, Eskeland T: The biology of macrophages: I. General principles and properties. Eur J Haematol, 51, 267-275, 1993.
2. Dalmo RA, Seljelid R: The immunomodulatory ef ect of LPS, laminaran and sulphated laminaran [β (1,3) - D - glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro. J Fish Dis, 18, 175-185, 1995.
3. Ainsworth AJ: A β-glucan inhibitable zymosan receptor on channel catfish neutrophils. Vet Immunol Immunopathol, 41, 141-152, 1994.
4. Figueras A, Santarem MM, Novoa, B: Inl uence of the sequence of administration of β-glucans and a Vibrio damsella vaccine on the immune response turbot (Scophthalmus maximus L.). Vet Immunol Immunopathol, 64, 59-68, 1998.
5. Kajita, Y, Sakai M, Atsuta SM: Immunopotentiationactivitiy of Freund’s Complete Adjuvant in rainbow trout, Oncorhynchus mykiss. JSFS, 58 (3): 433-437,1992.
6. Matsuyama H, Remy EP, Yano T: Protective ef ect of schizophyllan and scleroglucan against Streptococcus sp. infection in yellowtail (Seriola quinqueradiata). Aquaculture, 101, 197-203, 1992.
7. Mulero V, Estaban MA, Mesenguer J: Ef ects of in vitro addition of exogenous vitamins C and E on gilthead seabream (Sparus aurata L.) phagocytes. Fish Physiol Biochem, 18, 399-407, 1998.
8. Siwicki AK, Anderson DP, Rumsey GL: Dietary intake of immuno- stimulants by rainbow trout ef ects non-specific immunity and protection against furuncluosis. Vet Immunol Immunopathol, 41, 125-139, 1994.
9. Sveinbjornsson B, Seljelid R: Aminated β-1,3-D - polyglucose activates salmon pronephros macrophages in vitro. Vet Immunol Immunopathol, 41, 113-123,1994.
10. Watanuki H, Sakai M, Takahashi A: Immunomodulatory ef ects of alpha melanocyte stimulating hormone of common carp (Cyprinus carpio L.). Vet Immunol Immunopathol, 91, 135-140, 2003.
11. Janssen PA: The levamisole story. Progress Drug Res, 20,347-383, 1976.
12. Renoux G: The general immunopharmacology of levamisole. Drugs, 19, 89-99, 1980.
13. Siwicki AK, Cossarini DM: Ef ect of levamisole on the lymphocyte and macrophage activity in carp (Cyprinus carpio). Anns Vet Res, 21, 95- 100, 1990.
14. Siwicki AK, Anderson DP, Dixon OW: In vitro immunostimulation of rainbow trout (Oncorhynchus mykiss) spleen cells with levamisole. Dev Comp Immunol, 14, 231-237, 1990.
15. Anderson DP, Jeney G: Immunostimulants added to injected Aeromonas salmonicida bacterin enhance the defense mechanisms and protection in rainbow trout (Oncorhynchus mykiss). Vet Immunol Imunopathol, 34, 379-389, 1992.
16. Baba T, Watase Y, Yoshinaga Y: Activation of mononuclear phagocyte function by levamisole immersion in Carp. JSFS, 59 (2): 301- 307, 1993.
17. Findlay VL, Munday BL: Immunomodulatory ef ects of levamisole on the nonspecific immune system of Atlantic salmon, Salmo salar L. J Fish Dis, 23, 369-378, 2000.
18. Ispir U, Dorucu M: A study on the ef ects of levamisole on immune system Rainbow trout (Oncorhynchus mykiss, Walbaum). Tr J Vet Anim Sci, 29, 1169-1176, 2005.
19. Mulero V, Estaban MA, Munoz J, Mesenguer J: Dietary intake of levamisole enhances the immune response and disease resistance of the marine teleost gilthead seabream (Sparus aurata L.). Fish Shellfish Immun, 8, 49-62, 1998.
20. Siwicki AK: Immunomodulating activity of levamisole in carp spawners, Cyprinus carpio L. J Fish Biol, 31 (Supp. A), 245-246, 1987.
21. Siwicki AK: Immunostimulating inl uence of levamisole on non- specific immunity in carp, Cyprinus carpio L. Dev Comp Immunol, 13, 87- 91, 1989.
22. Cuesta A, Esteban MA, Meseguer J: Levamisole is a potent enhancer of gilthead seabream natural cytotoxic activity. Vet Immunol Immunopathol, 89, 169-174, 2002.
23. Anderson DP, Moritoma T, De Grooth R: Neutrophil, glass - adherent, nitroblue tetrazolium assay gives early indication of immunization ef ectiveness in rainbow trout. Vet Immunol Immunopathol, 30, 419-429, 1992.
24. Sövenyi A, Kusuda R: Kinetics of in vitro phagocytosis by cells from head-kidney of common carp, Cyprinus carpio. Fish Pathol, 22, 83-92, 1987.
25. Symoens J, Rosenthal M: Levamisole in the modulation of the immune response: the current experimental and clinical state. J Reticuloendothel Soc, 21, 175-221,1977.
26. Kimball ES, Schneider CR, Fisher MC, Clark MC: Levamisole causes dif erential cytokine expression by elicited mouse peritoneal macrophages. J Leukocyte Biol, 52, 349-356, 1992.
27. Tempero MA, Haga Y, Sivinsk C, Birt D, Klassen L, Thiele G: Immunologic effects of levamisole in mice and humans: evidence for augmented antibody response without modulation of cellular cytotoxicity. J Immunother, 17, 47-57,1995.
28. Jeney G, Anderson DP: An in vitro technique for surveying immuno- stimulants in fish. Aquaculture, 112, 283-287, 1993.
29. Mulero V, Estaban MA, Mesenguer J: In vitro levamisole fails to increase seabream (Sparus aurata L.) phagocyte functions. Fish Shellfish Immun, 8, 315-318, 1998.
30. Kimball ES: Experimental modulation of IL - I production and cell surface molecule expression by levamisole. ANN NY ACAD SCI, 685, 259- 268,1993.