Effat BEMANI, Saleh ESMAEILZADEH, Darioush GHARIBI, Masoud GHORBANPOOR

Immunohistochemical and Bacteriological Investigations of Mannheimia haemolytica in Sheep Bronchopneumonia

Mannheimia haemolytica enfeksiyonu tüm dünyada koyun pnömonilerinin en yaygın etiyolojik etkenidir. Ovine pnömonik mannheimiozis fibrinli pleuroplnömoni ile karakterizedir. Subakut ve kronik vakalarda purulent bronkopnömoni gelişir ve takibinde abseleşme ve fibrinli plöral yapışma şekillenir. Bu çalışmada, 8986 koyuna ait akciğer Ahvaz kesimevinde makroskopik olarak incelendi ve görsel olarak bronkopnömoni tespit edilen toplam 65 akciğer patolojik ve bakteriyolojik inceleme için seçildi. Mannheimia haemolytica antijenleri immunperoksidaz boyalı doku kesitlerinde %63.07 oranında belirlenirken örneklerin %52.30'u bakteriyolojik kültür ile pozitif olarak tespit edildi. Suppuratifi nekrotik ve fibrinli tipteki bronkopnömoniler en yaygın belirlenen lezyonlar iken sağ kranial loblar ve özellikle de onların kranial lopları en çok etkilenen bölgeler olarak belirlendi. McNemar testi immunohistokimya (İHK) ile bakteriyolojik kültür yöntemlerinin M. haemolytica (?=0.66) etkenini tespit etme güçleri arasında anlamlı bir fark olduğunu gösterdi. İHK yöntemi altın test olarak düşünüldüğünde bakteriyolojik kültür yönteminin özgüllüğü ve özgünlüğü sırası ile %78.05 ve %91.67 olarak belirlendi. Ki-kare testi lezyonların yayılımı ile bakteriyal izolasyon arasında (P=0.04), lezyon tipi ile IHK sonuçları arasında (P=0.01) ve bronkopnömoni tipi ile miks/saf izolasyon arasında (P=0.008) ilişki olduğunu gösterdi. Bu çalışma incelenen koyunlarda pnömonik lezyonların şekillenmesinde Mannheimia haemolytica'nın önemli rol oynadığını göstermektedir.

Koyun Bronkopnömonilerinde Mannheimia haemolytica'nın İmmunohistokimyasal ve Bakteriyolojik İncelenmesi

Mannheimia haemolytica infection is one of the most common etiologic agents of sheep pneumonia almost all over the world. Ovine pneumonic Mannheimiosis is characterized by severe fibrinous pleuropneumonia. Subacute to chronic cases progress to purulent bronchopneumonia and its squeals include abscessation and fibrous pleural adhesions. In the present study, lungs of 8986 sheep were inspected grossly in the Ahvaz abattoir and totally 65 lungs with visible signs of bronchopneumonia were selected for pathological and bacteriological examinations. Mannheimia haemolytica antigens were detected in 63.07% of immunoperoxidase stained tissue sections while 52.30% of the lungs were positive in bacteriological culture. Suppurative, necrotic and fibrinous types of bronchopneumonia were the most abundant lesions and right cranial lobes, specifically their cranial portions, were the most affected areas. McNemar test showed a significant difference between the diagnostic power of immunohistochemistry (IHC) and bacterial culture in detection of M. haemolytica (?=0.66). Considering IHC as a golden test, sensitivity and specificity of bacterial culture were estimated as 78.05 and 91.67%, respectively. Chi- squared test showed significant correlations between the distribution of the lesions and bacterial isolation (P=0.04), types of lesions and IHC results (P=0.01), and also types of bronchopneumonia and mixed/pure isolation (P=0.008). This study showed the significant role of Mannheimia haemolytica in causing pneumonic lesions of studied sheep.

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