SEMRA ÖZÇELİK, ÖMER POYRAZ, KADİR KALKAN, Erdoğan MALATYALI, Serpil DEĞERLİ

Hayvancılıkla Uğraşanlarda ve Sığırlarda Cryptosporidium spp. Yaygınlığının ELISA ile Araştırılması

Cryptosporidium spp. insanlarda ve hayvanlarda enterit oluşturan önemli bir etkendir. Çalışmada, zoonotik özelliği nedeniyle hayvancılıkla uğraşan kişilerde, şehir merkezinde yaşayan kontrol grubunda ve sığırlarda Cryptosporidiosis yaygınlığının araştırılması amaçlanmıştır. Eylül 2009- Eylül 2010 tarihleri arasında Sivas'a bağlı farklı köylerde yaşayan 50 çiftçiden, şehir merkezinde yaşayan, ishal v.b. yakınmaları olmayan 65 kişiden ve bu yörelerde yetiştirilen 200 sığır ve buzağıdan alınan dışkı örnekleri incelenmiştir. Toplanan örnekler çalışılıncaya kadar %10'luk formalin içinde saklanmıştır. Deneylerde Cryptosporidium Stool Antigen Microwell ELISA Kiti kullanılmış olup, çalışma sonucunda kontrol grubunda 4 (%6.2) kişide, hayvancılıkla uğraşan 9 (%18.0) kişide (?2=3.95, P

The Investigation of Cryptosporidium spp. Prevalence in Cattle and Farmers by ELISA

Cryptosporidium spp. is an important cause of enteritis for animals and humans. As the parasite has zoonotic potential, it is aimed to investigate the prevalence in people who had close contact with livestock, in control group who lived in Centrum and cattle. Fecal samples collected from 50 farmers who lived in different villages of Sivas and 65 people to be control group lived in Sivas Center without gastrointestinal discomfort and 200 cattle and calf breeding different county during the period from September 2009 to September 2010. Fecal samples were stored in 10% formalin until examination and then studied with Cryptosporidium Stool Antigen Microwell ELISA Kit. As a result, positive rate were found 4 (6.2%) in control group and 9 (18%) in study group (?2=3.95, P<0.05). On the other hand, 15 (7.5%) of the cattle and calve samples gave positive reaction with Cryptosporidium spp. ELISA

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