Ersen ERASLAN, Ayhan TANYELİ, Mustafa Can GÜLER, Nurhan AKARAS, Nezahat KURT

Gossypin Sıçanlarda Böbrek İskemi- Reperfüzyon Hasarına Karşı Korur

Böbrek iskemi reperfüzyonu sonucu meydana gelen renal hasar, böbrek yetmezliğine ve hatta ölüme neden olabilir. Çalışmanın amacı sıçan böbrek dokusunda iskemi-reperfüzyona bağlı oluşan doku hasarına karşı gossypinin olası koruyucu etkilerini araştırmaktır. Çalışmada toplam 48 adet Wistar albino cinsi erkek sıçan kullanıldı. Sıçanlar randomize ve eşit olmak üzere 6 gruba ayrıldı (n=8). Kontrol (C), sham (S), istemi-reperfüzyon (I/R), I/R + DMSO, I/R + 400 μg/kg gossypin ve I/R + 4 mg/kg gossypin grupları oluşturuldu. Sham grubunda arka bölge açılarak sağ nefrektomi yapıldı. Gossypin reperfüzyondan önce intraperitoneal olarak uygulandı. Sol renal artere 1 saat iskemi sonunda 24 saat reperfüzyon uygulandı. Prosedürler tamamlandıktan sonra böbrek dokularında spektrofotometrik metodlarla TNF-α, IL- 1β, IL-6 and IL-10 seviyeleri ölçüldü. Apoptozis ve inflmatuvar yolaklar kazpaz-3 ve NF-κB antikorları kullanılarak değerlendirildi. Gossypin grupları I/R grupları ile kıyaslanınca IL-1β and IL-6 seviyelerinde istatistiksel olarak önemli derecede azalma tespit edildi (P

Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats

Renal injury occurring as a result of renal ischemia-reperfusion may lead to renal failure or even death. The aim of this study is to investigatepossible protective effcts of Gossypin on tissue damage occurred due to ischemia-reperfusion in rat kidney tissue. A total of 48 male Wistaralbino rats were used in the study. These rats were randomly divided into 6 groups equally (n = 8). The created groups were control (C),sham (S), ischemia-reperfusion (I/R), I/R + DMSO, I/R + 400 μg/kg gossypin and I/R + 4 mg/kg gossypin. In the rats of sham group, the rightnephrectomy was performed. In the rats of other groups rather than sham, the left renal artery was clamped after performing the rightnephrectomy. Gossypin was administered intraperitoneally before the reperfusion. 24 h reperfusion was applied to the left renal after 1 hof ischemia. TNF-α, IL-1β, IL-6 and IL-10 levels were measured with spectrophotometric methods in the kidney tissues after the procedureswere completed. Apoptosis and inflmmatory pathways were evaluated histopathologically using Caspase 3 and NF-κB antibodies. Therewas a statistically signifiant decrease in IL-1β and IL-6 levels of the gossypin groups compared to the I/R group (P

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