SİNAN İNCE, AYHAN FİLAZİ

Farelerde korunga bitkisinin (Onobrychis viciifolia) bağırsaklara etkisi

Bu araştırmada, korunga bitkisi (Onobrychis viciifolia) ekstraktının Wistar albino soyu en az iki aylık fare jejunum ve ileumu üzerindeki etkilerinin izole organ banyosunda in vitro testlerle ortaya konması amaçlanmıştır. Jejunum ve ileum dokusunda, asetilkolin EC50, betanekol EC50, atropin (10-8-10-7 M) ve korunga ekstraktı 0.1-6.4 mg/ml derişimlerde uygulanarak tek başlarına ve bir arada doku üzerindeki etkileri incelendi. Ayrıca histamin ve nikotinin jejunum ve ileum üzerindeki etkileri incelendi. Ekstraktın (6.4 mg/ml) fare jejunum ve ileum düz kası üzerinde gevşetici etki yaptığı, asetilkolin EC50 ve betanekol EC50’nin ekstrakt (3.2 ve 6.4 mg/ml) varlığında kastırıcı etkilerinin, atropin varlığında ekstraktın (6.4 mg/ml) gevşetici etkisinin azaldığı ve böylece bitkinin antimuskarinik ajan olarak rol oynadığı belirlendi. Bitki ekstraktının bağırsaklar üzerindeki etkilerini reseptörler düzeyinde oluşturduğu, bu etkinin görülmesinde içeriğinde bulunan etkin maddelerin özellikle de flavonoidlerin rol oynadığı sonucuna varılmıştır.

Anahtar Kelimeler:

ileum, yem bitkileri, Onobrychis viciifolia, flavonoidler, ince bağırsak, bitki özütü, fareler, bağırsaklar

The effects of sainfoin (Onobrychis viciifolia) on intestine in mice

In this study, it was aimed to determine the effects of sainfoin (Onobrychis viciifolia) extract on jejunum and ileum smooth muscle of Wistar albino mice of at least 2 months age in vitro by using an isolated organ bath. The effects of aceytlcholine EC50, bethanechol EC50, atropine (10-8-10-7 M) and 0.1-6.4 mg/ml concentration of the sainfoin extract were administrated alone and in combinations to jejunum and ileum. Furthermore, the effects of histamine and nicotine on jejunum and ileum were studied. Extract (6.4 mg/ml) has inhibitory effects on the smooth muscles of mice jejunum and ileum; the contractions of aceytlcholine EC50 and bethanechol EC50 was decreased by the presence of the extract at 3.2 and 6.4 mg/ml concentrations. The inhibitory effects of the extract at 6.4 mg/ml concentration was decreased by the presence of atropine. These results indicate that this plant acts as an antimuscarinic agent. The effects of the plant extract on the receptors of intestine were probably mediated due to its active components, especially flavonoids.

Keywords:

ileum, fodder plants, Onobrychis viciifolia, flavonoids, small intestine, plant extracts, mice, intestines,

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