Sirri KAR, ESİN GÜVEN, NADİM YILMAZER, Zafer KARAER

Determination of developmental stages of Cryptosporidium parvum in HCT-8 cell culture by differential interference contrast microscopy, Giemsa and haematoxylin-eosin staining methods

Bu çalışmada, interferans kontrast mikrsokopisi, Giemsa ve hematoksilin-eosin boyama yöntemlerinin, Cryptosporidium parvum’un hücre kültüründeki gelişme evrelerinin belirlenmesi konusundaki yetilerinin karşılaştırılması amaçlanmıştır. Elde edilen veriler, interferans kontrast mikroskopisi ve Giemsa boyama yönteminin söz konusu parazitin gelişme evrelerinin belirlenmesinde bazı önemli avantajlara sahip olduğunu, formalin ile tespit işlemini takiben uygulanan hematoksilin-eosin boyama tekniğinin ise parazitte meydana gelen bazı değişikliklerin kolayca belirlenmesini sağladığını göstermiştir. Taramalarda, interferans kontrast mikroskopisi ile meront ve mikrogametlerin kesin olarak ayırt edilebileceği, boyama yöntemleriyle ise sadece merontların belirlenebileceği anlaşılmıştır.

Anahtar Kelimeler:

Cryptosporidium parvum, giemsa boyaması, hücre kültürü, eozin, interferans, mikroskopla inceleme, ookist

Cryptosporidium parvum’un HCT-8 Hücre Kültüründeki gelişme evrelerinin diferansiyel interferans kontrast mikroskopisi, Giemsa ve hematoksilin-eozin boyama yöntemleriyle belirlenmesi

This study aimed to compare the advantages of diff erential interference contrast microscopy, Giemsa and haematoxylin-eosin staining techniques for detecting, visualizing and distinguishing the developmental stages of Cryptosporidium parvum in cell culture. Data showed that interference contrast microscopy and Giemsa staining have certain advantages, whereas haematoxylin-eosin staining followed by formalin fi xation clearly reveals certain changes occurred in the parasite. In the investigations, it was revealed that direct microscopy is eff ective in accurate defi nition of meronts and microgamets, but staining methods can diff erentiate meronts only.

Keywords:

Cryptosporidium parvum, Giemsa staining, cell culture, eosine, interference, microscopy, oocysts,

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