Maryam KARIMI DEHKORDI, Ali KADIVAR, Taghi TAKTAZ HAFSHEJANI

Critical Thresholds of Nonesterified Fatty Acids and ?-hydroxybutyrate in Transition Dairy Cows for Prediction of First Service Conception Rate

Bu çalışmanın amacı ineklerde ilk tohumlamada gebe kalma oranını tahmin etmede ?-hidroksibütrat (BHBA) ve esterlenmemiş yağ asitleri (NEFA) kritik eşik değerlerini belirlemektir. Çalışmanın verileri ticari büyük bir çiftlikte 97 adet Holstein ırkı inekten (2 ile 5 parite) elde edildi. Tüm ineklerde BHBA ve NEFA serum konsantrasyonları prepartum 10. günde ve postpartum 1, 2, 4 ve 6. haftalarda ölçüldü. BHBA ve NEFA ilk tohumlamada gebe kalma eşik değerini belirlemek amacıyla Receiver Operator Characteristic (ROC) ile analiz edildi. Postpartum 4. ve 6. haftalardaki NEFA ROC analizinde belirlenen tek anlamlı değerlerdi. 4. ve 6. haflarda NEFA için optimal kritik eşik değerleri sırası ile 201.15 µmol/L ve 203.4 µmol/L olarak tespit edildi (P=201.15 ineklerde (OR=0.176; P=0.001) %82.4 ve 6. haftada NEFA >=203.4 µmol/L ineklerde (OR=0.114; P=0) %88.6 daha düşük olarak belirledi. Sonuç olarak, doğumdan sonra 4 ve 6. haftalarda NEFA konsantrasyonları ilk suni tohumlamada gebe kalma olasılığı ile düşük derecede ilişkilidir.

Geçiş Dönemi Sütçü İneklerde İlk Tohumlamada Gebe Kalma Oranını Tahmin Etmede Esterlenmemiş Yağ asitleri ve ?-hidroksibütirat Kritik Eşik Değerleri

The objective of this study was to establish cow level critical thresholds for ?-hydroxybutyrate (BHBA) and nonesterified fatty acids (NEFA) to predict conception to first service. The data were generated from 97 Holstein cows (2 to 5 parities) on a large commercial farm. Serum concentrations of BHBA and NEFA were measured in all cows on day 10 prepartum and, weeks 1, 2, 4 and 6 postpartum. NEFA and BHBA analyzed with receiver operator characteristic (ROC) analysis to determine critical thresholds for predicting pregnancy to first service. NEFA in weeks 4 and 6 postpartum were the only significant predictors identified in the ROC analysis. Optimum critical thresholds for NEFA in weeks 4 and 6 were 201.15 µmol/L and 203.4 µmol/L, respectively (P<0.05). The critical threshold for serum BHBA in the prepartum cohort was 600 µmol/L (P=0.1), which predicted conception to first service. Logistic regression analysis indicated that the risk of conceiving was 82.4% and 88.6% lower for cows with NEFA >=201.15 in week 4 (OR=0.176; P=0.001) and NEFA >=203.4 µmol/L in week 6 (OR=0.114; P=0), respectively. In conclusion, NEFA concentrations within 4 and 6 weeks after calving were associated with lower probability of pregnancy at the first AI.

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