Bazı sinek (Dizi: Diptera) türlerinde Wolbachia spp’nin PZR ile araştırılması

Wolbachia spp., Riketsia soyundan gram negatif hücre içi bir bakteri olup, arthropodlarda yatay ve dikey olarak taşınmakta, üreme ile ilgili dokularında da bulunmaktadırlar. Bu çalışmanın amacı farklı tür erkek ve dişi sineklerde Wolbachia spp.’nin PZR ile aranmasıdır. Bu amaçla 33 dişi ve 15 erkek Musca spp., 30 dişi ve 7 erkek Lucilia sericata, 16 dişi ve 4 erkek Calliphora vicina, 1 dişi ve 4 erkek Sarcophaga haemorrhoidalis ile 27 dişi ve 10 erkek Chrysomia albiceps kullanılmıştır. Genomik DNA izolasyonundan sonra Wolbachia wsp (Wolbachia surface protein) genini çoğaltan spesifik primerler ile PZR yapılmıştır. Çalışma neticesinde pozitif kontrol örneğinde 630 bp’lik band gözlenirken, negatif kontrol ve test edilen hiçbir örnekte pozitiflik belirlenememiştir. Sonuç olarak bu çalışma ile Türkiye’de ilk kez bazı Diptera populasyonlarında Wolbachia spp. moleküler olarak araştırılmıştır.

Investigation of Wolbachia spp in some flies species (Order: Diptera) by PCR

Wolbachia is a gram negative intracellular parasite from Rickettsia family which is located into reproductive organs of arthropods and transmitted by horizontally and vertically. The aim of this study was to investigate of Wolbachia spp. in different species of flies by PCR. For this aim, 33 females and 15 males of Musca spp., 30 females and 7 males of Lucilia sericata, 16 females and 4 males of Calliphora vicina, 1 female and 4 males of Sarcophaga haemorrhoidalis and 27 females and 10 males of Chrysomia albiceps adult flies were used. After the isolation of genomic DNA, the specific primers were used and PCR was performed for the amplification of Wolbachia wsp (Wolbachia surface protein) gene. As a result, the positive control sample yielded 630 bp band while negative control and none of the tested samples yielded band. In conclusion, Wolbachia spp has been molecularly investigated in some Diptera populations for the first time in Turkey.

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