Optimization of Hydrophilic-Lipophilic Balance Solid-Phase Extraction of Phthalates in Pharmaceutical Preparations
Phthalates, which are used as plasticizers to soften rough polymers in the industrial processes, possess endocrine system disrupting activities. In this study, a hydrophilic-lipophilic balance solid-phase extraction method was optimized for seven phthalates as dimethyl, dipropyl, benzyl butyl, di-n-butyl, dicyclohexyl, di-(2-ethylhexyl) and di-n-octyl phthalates in terms of the type and the volume of the conditioning and elution solvents. The phthalates were quantified by a validated HPLC/UV method. The recovery values were higher than 80% for dimethyl, dipropyl, benzylbutyl, di-n-butyl and dicyclohexyl phthalates. Using the optimized conditions three types of liquid pharmaceutical preparations as an intravenous isotonic sodium chloride solution, an intravenous dextrose solution and an osmotic laxative kept in polymeric packages were analyzed with high accuracy, precision and recovery. Only the intravenous isotonic sodium chloride solution was determined to be contaminated with dipropyl phthalate at a concentration of 13.2 μg/L.
Keywords:
Hydrophilic-lipophilic balance, high performance liquid chromatography, pharmaceutical, phthalate solid-phase extraction,
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- 1. Gómez-Hens A, Aguilar-Caballos M. Social and economic interest in the control of phthalic acid esters. TrAC Trends in Analytical Chemistry. 2003 Dec;22(11):847–57.
- 2. Yılmaz PK, Ertaş A, Kolak U. Simultaneous determination of seven phthalic acid esters in beverages using ultrasound and vortex-assisted dispersive liquid–liquid microextraction followed by high-performance liquid chromatography. Journal of Separation Science. 2014 Aug;37(16):2111–7.
- 3. Ghisari M, Bonefeld-Jorgensen EC. Effects of plasticizers and their mixtures on estrogen receptor and thyroid hormone functions. Toxicology Letters. 2009 Aug;189(1):67–77.
- 4. Russo MV, Avino P, Perugini L, Notardonato I. Extraction and GC-MS analysis of phthalate esters in food matrices: a review. RSC Advances. 2015;5(46):37023–43.
- 5. Brossa L, Marcé R., Borrull F, Pocurull E. Application of on-line solid-phase extraction–gas chromatography–mass spectrometry to the determination of endocrine disruptors in water samples. Journal of Chromatography A. 2002 Jul;963(1–2):287–94.
- 6. Del Carlo M, Pepe A, Sacchetti G, Compagnone D, Mastrocola D, Cichelli A. Determination of phthalate esters in wine using solid-phase extraction and gas chromatography–mass spectrometry. Food Chemistry. 2008 Dec;111(3):771–7.
- 7. Guo Z, Wang S, Wei D, Wang M, Zhang H, Gai P, et al. Development and application of a method for analysis of phthalates in ham sausages by solid-phase extraction and gas chromatography–mass spectrometry. Meat Science. 2010 Mar;84(3):484–90.
- 8. Sánchez-Avila J, Fernandez-Sanjuan M, Vicente J, Lacorte S. Development of a multi-residue method for the determination of organic micropollutants in water, sediment and mussels using gas chromatography–tandem mass spectrometry. Journal of Chromatography A. 2011 Sep;1218(38):6799–811.
- 9. González‐Sálamo J, Hernández‐Borges J, Afonso M del M, Rodríguez‐Delgado MÁ. Determination of phthalates in beverages using multiwalled carbon nanotubes dispersive solid-phase extraction before HPLC–MS. Journal of Separation Science. 2018 Jun;41(12):2613–22.
- 10. Luo Y-B, Yu Q-W, Yuan B-F, Feng Y-Q. Fast microextraction of phthalate acid esters from beverage, environmental water and perfume samples by magnetic multi-walled carbon nanotubes. Talanta. 2012 Feb;90:123–31.
- 11. Ye Q, Liu L, Chen Z, Hong I. Analysis of phthalate acid esters in environmental water by magnetic graphene solid phase extraction coupled with gas chromatography–mass spectrometry. Journal of Chromatography A. 2014 Feb;1329:24–9.
- 12. Tolmacheva VV, Apyari VV, Kochuk EV, Dmitrienko SG. Magnetic adsorbents based on iron oxide nanoparticles for the extraction and preconcentration of organic compounds. Journal of Analytical Chemistry. 2016 Apr;71(4):321–38.
- 13. Chen N, He J, Wu C, Li Y, Suo A, Wei H, et al. Synthesis of molecularly imprinted polymers by atom transfer radical polymerization for the solid-phase extraction of phthalate esters in edible oil. Journal of Separation Science. 2017 Mar;40(6):1327–33.
- 14. Guart A, Bono-Blay F, Borrell A, Lacorte S. Migration of plasticizersphthalates, bisphenol A and alkylphenols from plastic containers and evaluation of risk. Food Additives & Contaminants: Part A. 2011 May;28(5):676–85.
- 15. Zdravkovic SA. Solid phase extraction in tandem with GC/MS for the determination of semi-volatile organic substances extracted from pharmaceutical packaging/delivery systems via aqueous solvent systems. Journal of Pharmaceutical and Biomedical Analysis. 2015 Aug;112:126–38.
- 16. Petruševski V, Jolevska ST, Ribarska JT, Chachorovska M, Petkovska A, Ugarković S. Development of complementary HPLC-DAD/APCI MS methods for chemical characterization of pharmaceutical packaging materials. Journal of Pharmaceutical and Biomedical Analysis. 2016 May;124:228–35.
- 17. Demoré B, Vigneron J, Perrin A, Hoffman MA, Hoffman M. Leaching of diethylhexyl phthalate from polyvinyl chloride bags into intravenous etoposide solution. Journal of Clinical Pharmacy and Therapeutics. 2002 Apr;27(2):139–42.
- 18. Mitani K, Izushi F, Kataoka H. Analysis of phthalate contamination in infusion solutions by automated on-line in-tube solid-phase microextraction coupled with high-performance liquid chromatography. J Anal Toxicol. 2004 Oct;28(7):575–80. 19. Chaudhary AK, Waske SA, Yadav S, Chandrashekhar TG, Singh V. Validated reverse phase HPLC method for the determination of DEHP content in reconstituting diluents and in reconstituted solutions of imipenem and cilastatin for injection. E-Journal of Chemistry. 2010;7(2):501-13.
- Başlangıç: 2014
- Yayıncı: Türkiye Kimya Derneği
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