Arzu ALTUNÇEKİÇ YILDIRIM, Selma Cırrık, Yeliz Çetinkol, Mustafa Kerem Çalgın, Tevfik Noyan

Kronik hepatit C hastalarında serum GRP78 düzeyleri anlamlı mı? Vaka-kontrol çalışması

Amaç: Glikozla düzenlenen protein 78 (GRP-78) dokudaki endoplazmik retikulum (ER) stresinin temel göstergelerinden birisidir. Hepatit C ile enfekte hastalarda karaciğerde ER stresinin geliştiği bilinmektedir. Bu çalışmada kronik hepatit C (KHC) hastalarında bir stres belirteci olarak daha önce incelenmemiş olan serum GRP78 düzeylerinin değerlendirilmesi amaçlanmıştır.Yöntemler: Çalışmamız, Enfeksiyon Hastalıkları polikliniğimize başvuran Kronik Hepatit C (KHC) enfeksiyonu tanısı almış hasta grubu (n=60) ve ek kronik hastalığı olmayan sağlıklı kontrol (n=60) grubundan oluşan bir vaka kontrol çalışmasıdır. Serum GRP78 seviyesi Enzyme-Linked Immuno Sorbent Assay (ELISA) ile ölçülmüş, ardından GRP78 ile alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), HCV-RNA düzeyleri arasında korelasyon analizi yapılmıştır.Bulgular: KHC hastalarında HCV-RNA düzeyleri ile ALT ve AST düzeyleri arasında anlamlı bir ilişki izlenmiştir (sırasıyla P<0,001 ve P=0,008). Serum GRP78 hem kontrol hem de HCV alt gruplarında benzer seviyelerde saptanmıştır. Serum GRP78 seviyesi ile AST düzeyleri arasında anlamlı bir pozitif korelasyon saptanırken (P=0,046), serum ALT düzeyleri ile anlamlı bir ilişki saptanamamıştır.Sonuç: HCV ile indüklenen karaciğer hasarında ER stresinin geliştiği gösterilmiş olmasına rağmen, sonuçlarımız kronik HCV enfeksiyonu sırasında serum GRP 78 düzeyinde anlamlı bir artış olmadığını göstermektedir.

Anahtar Kelimeler:

Glikozla düzenlenen protein78, Hepatit C enfeksiyonu, Endoplazmik retikulum stres

Are serum GRP78 levels significant in chronic hepatitis C patients? A case-control study

Aims: Glucose-regulated protein 78 (GRP-78) is one of the basic markers of endoplasmic reticulum (ER) stress in tissues. It is known that ER stress develops in the livers of patients infected with hepatitis C. In this study, the aim was to assess serum GRP78 levels which have not previously been investigated as a stress marker in chronic hepatitis C patients (CHC).Methods: This case control study includes patients with chronic hepatitis C (CHC) infection in our Infectious Diseases clinic (n=60) and a healthy control group without any additional chronic disease (n=60). Serum GRP78 levels were measured with enzyme-linked immunosorbent assay (ELISA), then correlation analysis was performed for serum GRP78 levels with alanine aminotransferase (ALT), aspartate aminotransferase (AST) and HCV-RNA levels.Results: A significant positive correlation was observed between HCV-RNA, ALT and AST levels in CHC patients (P<0.001 and P=0.008, respectively). Serum GRP78 was identified at similar levels in both the control and HCV subgroups. While a significant positive correlation was identified between serum GRP78 and AST levels (P=0.046), no significant correlation was detected for serum ALT levels.Conclusion: Though liver injury induced by HCV is shown to cause ER stress, our results showed there was no significant increase in serum GRP78 levels during chronic HCV infection.

Keywords:

Glucose-regulated protein 78, Hepatitis C infection, Endoplasmic reticulum stress,

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