Microscopic identification of Echinops spinosus ssp.bovei (Boiss.) Murb. using multivariate tests
Microscopic identification of Echinops spinosus ssp.bovei (Boiss.) Murb. using multivariate tests
The genus Echinops (Asteraceae family, Echinopeae class) comprises ca. 120 species and is native to Africa, the Middle East, Europe, and Asia. In Algeria, this genus is represented by the very common species Echinops spinosus L., also known as “Tesskra”, which is used as a diuretic, hypoglycemic, liver disorders, for post-partum care, and for its stomachic effects. The aim was to generate microscopic parameters of the aerial parts which could be used to identify and authenticate Echinops powders. The present study reports, for the first time, the detailed pharmacognostic characters of the different parts of E. spinosus, through microscopic approaches, coupled with statistical techniques, to analyze the structural observations. Multivariate tests were used to determine the complex relationships among the studied variables. Fifty observations were analyzed from the leaves, flowers, and roots of E. spinosus. As a result, fourteen key microscopic features were identified and analyzed by correspondence analysis. The sclereid fibers were positively linked on the first factor (0.600, 0.591), but weakly related to the second factor (0.090, 0.004). It is proposed that microscopic analysis, coupled with statistical analysis, could provide a simple platform for medicinal plant identification. This will be of particular importance for the rapid identification of medicinal plant powders used as a medicinal agent, and may be helpful in developing pharmacopoeial standards for traditional medicines in Africa.
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- Halim AF, Afify MS, Ahmed AF, Mira AS. The fact about Echinopsine and first isolation of echinorine from Echinops spinosus L. J Environ Sci. 2011; 40(2): 173-181.
- Bellakhdar J, Claisse R, Fleurentin J, Younos C. Repertory of standard herbal drugs in the Moroccan pharmacopoea. J Ethnopharmacol. 1991; 35(2): 123-143. [CrossRef]
- Baghdad M, Boumediene M, Djamel A, Djilali B. Ethnobotany of medicinal plants in the region Béni chougrane (Mascara, Algeria). J Bio Environ Sci. 2016; 9(1): 426-433.
- Dobignard A, Chatelain C, Index synonymique de la flore d’Afrique du Nord, second ed., Conservatoire et Jardin Botanique, Genève 2011.
- Kiyekbayeva LN, Akhtaeva NZ, Datkhayev UM, Omarkhan AB, Litvinenko YA, Tynybekov BM, Berkenov AK. Pharmacognosy signs of aerial parts of medicinal plant Echinops albicaulis Kar et Kir. Asian Pharm Clin Res. 2017; 10(6): 346-348. [CrossRef]
- Zhao ZZ, Hu YN, Wong YW, Wong WCG, Wu K, Jiang ZH, Kang T. Application of Microscopy in Authentication of Chinese Patent Medicine-Bo Ying Compound. Microsc Res Techniq. 2005; 67: 305-311. [CrossRef]
- The Pharmacopoeia Committee of the Ministry of Health of the People Republic of China, Pharmacopoeia of the People’s Republic of China, vol. 1, People’s Heath Publishing House, Chemical Industry Publishing House, Beijing 2000.
- Kang TG, Microscopic identification of Chinese Material Medica, China Press of Traditional Chinese Medicine, Shanghai, China 2003.
- State Administration of Chinese Medicine, «Chinese materia medica (I–IX)», Shanghai. Science and Technology Publishing House, Shanghai, China 1999.
- Xiao PG, ‘‘New compendium of Chinese Materia Medica (I–IV)’’, Chemical Industry Press, Beijing, China 2002.
- British Herbal Medicine Association, British Herbal Medicine Pharmacopoeia, Biddles Ltd., London 1996.
- Upton R, American herbal pharmacopoeia and therapeutic compendium, Astragalus root, Santa Cruz 1999a.
- Upton R, American herbal pharmacopoeia and therapeutic compendium, Schisandra berry, Santa Cruz 1999b.
- Upton R, American herbal pharmacopoeia and therapeutic compendium, Reishi mushroom, Santa Cruz 2000.
- Upton R, American herbal pharmacopoeia and therapeutic compendium, Dang gui root, Santa Cruz 2003.
- Society of Japanese Pharmacopoeia, Japanese pharmacopoeia (English version), fourteenth ed., Junkudo Book Shop, Tokyo, Japan 2001.
- Ministry of Health & Family Welfare of South Korea, Korea Herbal Pharmacopoeia, Korea 2002.
- Ministry of Health & Family Welfare of India, The ayurvedic pharmacopoeia of India, third vol., The Controller of Publications Civil Lines, Delhi, India 2001.
- Lachumy SJ, Sasidharan S. The usage of microscopy method for herbal standardizations. In: Méndez-Vilas A. Current Microscopy Contributions to Advances in Science and Technology. Formatex Research Center, Spain, 2012, pp. 704710.
- Bouzabata A. Microscopic analysis of Curcuma longa using multivariate test. Int J Pharmacognosy. 2015; 2(4): 173-177.
- Indika Chandralal Herath HM, Sirimal Premakumara GA, Wijayasiriwardene CMK. Morphological and microscopic analysis of five curcuma species grown in Sri Lanka using multivariate test. Int J Pharmacognosy. 2017; 4(7): 224-231.
- World Health Organization (WHO), Monographs on Selected Medicinal Plants, first vol., Geneva 1999.
- Sharma V, Pracheta V. Microscopic studies and preliminary pharmacognostica evaluation of Euphorbia neriifolia L. leaves. Indian J Nat Prod Res. 2013; 4(4): 348-357.
- Wichtl M, Anton R. Plantes Thérapeutiques: Tradition, Pratique Officinale, Science et Thérapeutique, Lavoisier, Strasbourg, France 1999.