Abdul-baki Abdullah AL-ROBASİ, Wedaad Dawood ROHAİM, Dheya Abdulhafeed AL-DANANİ

Seroprevalence of Leptospira antibodies among populations at risk

Amaç: Bu çalışma risk altındaki insanlardaki Leptospira IgG antikorlarının seroprevalansını değerlendirmek amacıyla Yemen’in Hudeyde Vilayetinde yapıldı.Yöntemler: Bu çalışma 2012 yılı Haziran ve Aralık ayları arasında yapıldı. Çalışmaya136 erkek ve 64 kadın olmak üzere toplamda 200 kişi katıldı. Çalışmaya katılanlar 10 kanalizasyon işçisi, 22 kasap, 16 inşaat işçisi, 108 tarım işçisi, 20 sağlık çalışanı ve 24 kan bağışçısından oluşmaktaydı. Gönüllü kişiler önceden hazırlanan anketi cevaplayarak çalışmaya katıldılar. Katılımcılardan kan örnekleri alındı ve bu örneklerde Leptospira IgG antikorlarının varlığı ELISA yöntemiyle test edildi. Seropozitiflik ile ilgili muhtemel risk faktörleri değerlendirildi.Bulgular: Leptospira antikorlarının risk altındaki toplumda sıklığı %42 olarak belirlendi. En yüksek seviye kanalizasyon işçilerinde %80 olarak tespit edildi. Kanalizasyon işçilerini sağlık çalışanları (%60), inşaat işçileri (%37,5) ve tarım çalışanları (%37) izledi. En düşük seviye kasaplarda görüldü (%36,4). Kan bağışçıları arasında seroprevalans %25 olarak bulundu ve bu da risk altındaki kişilere göre düşüktü. Leptospira IgG seropozitifliği erkeklerde kadınlara göre daha yüksekti (%42,6’ya karşılık %34,4). En yüksek Leptospira antikor serapositifliği %81,8 ile 58 yaş ve üzeri kişilerde görüldü. Kırsal alanda yaşayanlardaki leptospira IgG antikor sıklığı ile kentsel alanda yaşayanlar arasında önemli bir fark yoktu. Hayvanlarla temas halinde olanlar arasında en yüksek antikor pozitifliği keçi besleyen kişilerde (%80) ve koyun besleyen kişilerde (%60,9) idi. Sonuç: Risk altında olan kişiler çoğunlukla leptospiroza maruz kalan kişilerdir. Bundan dolayı kontrol stratejilerinin bu kişileri hedef alması gereklidir

Anahtar Kelimeler:

Leptospira antikorları, seroprevalans, riskli gruplar

Seroprevalence of Leptospira antibodies among populations at risk

Objective: This study was performed to assess the Leptospira IgG antibodies seroprevalence among populations at risk in Hodeida Governorate, Yemen. Methods: A total of 200 subjects (136 males and 64 females) participated in this study during June and December 2012.They represented 10 sewage workers, 22 butchers, 16 construction workers, 108 agriculture workers, 20 hospital sanitary workers and 24 blood donors. Predesigned questionnaires and consent were taken from each individual. Blood samples were collected from subjects, and the sera were tested by ELISA to detect the presence of leptospira IgG antibodies. The possible related factors for seropositivity were evaluated. Results: Leptospira IgG antibodies were found positive in 42% of the participants. The highest seroprevalence level was detected in sewage workers (80%), followed by hospital sanitary workers (60%), construction workers (37.5%) and farmers (37%). The lowest of antibodies was in butchers (36.4%). Seroprevalence among blood donors was 25% which was comparatively less than of the populations at risk. Seropositivity of Leptospira IgG antibodies was found higher among males than females (42.6% vs. 34.4%). The highest Leptospira antibodies seropositivity was among elderly participants (81.8%). The seropositivity of antibodies in population live in rural and urban areas was not significant differences. As for closely contacting with animals, the highest antibodies were discovered in people who had goats (80%) and sheep (60.9%). Conclusion: Individuals engaged in risk activities are often exposed to leptospiral infection. Therefore, control and prevention policy toward these people are necessary.

Keywords:

Leptospira antibodies, seroprevalence, risk groups,

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Adler B. History of leptospirosis and leptospira. Curr Top Mi- crobiol Immunol. 2015;387:1-9.
World Health Organization. Reports of the first meeting of the Leptospirosis burden epidemiology reference group. WHO, Geneva, Switzerland 2010; pp. 1-40.
Russeli KL, Gonzalez MAA, Watts DM, et al. An outbreak of Leptospirosis among Peruvian Military Recruits. Am J Trop Med Hyg 2003;69:53-57.
Noor Rafiz AA, Aziah BD, Azwany YN, et al. Leptospirosis in northeastern Malaysia: Misdiagnosis or Confection. Intern J Cola Resear Internal Med Pub Heal 2012;4:1419-1427.
Zavala-Velazquez J, Cardenas-Marrufo M, Vado-Solis I, et al. Hemorrhagic pulmonary leptospirosis: three cases from the Yucatan Peninsula, Mexico. Rev Soc Bras Med Trop 2008;41:404-408. Available from: www. Scielo.br. (Accessed January 2015).
Brown MG, Vickers IE, Salas RA, et al. Leptospirosis in sus- pected cases of dengue in Jamaica, 2002-2007. Tropical doct 2010;40:92-94.
Sambasiva RR, Naveen G, Bhalla P, et al. Leptospirosis in India and the rest of the word. Braz J Infect Dis 2003;7:178- 193.
Vimala G, Rani AMJ, Gopal VR. Leptospirosis in Vellore: A clinical and serological study. Int J Microbiol 2014;643940.
Thipmontree W, Suputtamongkal Y, Tantibhyanghul W, et al. Human leptospirosis Trends: Northeast Thailand 2001-2012. Int J Environ Res Public Health 2014;11:8542-8551.
Laras K, Van C, Bounlu K, et al. The importance of leptospiro- sis in Southeast Asia. Am J Trop Med Hyg 2002;67:278-286.
Yimer E, Koopman S, Messele T, et al. Human leptospirosis in Ethiopia: A pilot study in Wonji, Ethiopian. J Health De- velop 2004;18:48-51.
Koteeswaran A. Seroprevalence of leptospirosis in man and animals in Tamilnada. India J Med Microbio 2006;24:329- 331.
Faragi H, Assmar M, Ebrahimipour GHH, et al. The survey of seroprevalence of leptospirosis in Mazandaran province of Iran using Microscopic agglutination test and indirect im- munofluorescence assay. Iranian J Biolog 2010;22:608-618.
Gongora A, Parra J, Aponte L, Gómez L. Seroprevalence of Leptospiraspp in population groups of Villavicencio, Colom- bia. Rev Salud Publica 2008;10:269-278.
Schoonman L, Swai ES. Risk factors associated with the se- roprevalence of leptospirosis, amongst at-risk groups in and around Tanga City. Ann Trop Med Parasitol 2009;103:711- 718.
Kawaguchi L, Sengkeopraseuth B, Tsuyuoka R, et al. Sero- prevalence of leptospirosis and risk factors analysis in flood- prone rural areas in Lao PDR. Am J Trop Hyg 2008;78:957- 961.
Esmaeili R, Hesamzadeh A, Alizadeh-Navaei R, et al. Inci- dence of leptospirosis in Mazandaran province, North of Iran: A one year survey. Pak J Biol Sci. 2009;12:1330-1333.
Zamora J, Riedemann S, Montecinos MI, Cabezas X. Sero- logical survey of human leptospirosis in a high risk popula- tion in Chile. Rev Med Chil 1990;118:247-252.
WHO SEARO organized an informal regional expert consul- tation on leptospirosis surveillance, diagnosis and risk reduc- tion in Chennai from. 17-18 October WHO, 2009. http://www. searo.who.int/entity/emerging_diseases/topics/Communi- cable_Diseases_Surveillance_and_response_SEA-CD-216. pdf
Swapna RN, Tuteja U, Nair L, Sudarsana J. Seroprevalence of leptospirosis in high risk groups in Calicut, North Kerala, India. Indian J Med Microbiol 2006;24:349-352.
Sharma S, Vijayachari P, Sugunan AP, Natarajaseenivasan K, Sehgal SC. Seroprevalence of leptospirosis among high- risk population of Andaman Islands, India. Am J Trop Med Hyg 2006;74:278-283.