Merwa Abdelazim MANSOUR, Tarik I. ZAHER, Amany M. IBRAHIM, Amira S. AHMED, Islam M. IBRAHIM, Ashraf GODA, Nehad F. AHMED

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Mısırlı hastalarda tifo taşıyıcılığında safra taşlarının rolü

Objectives: Salmonella typhi forms biofilm on the surface of gallstones promoting colonization and carrier state. The aims are to detect its frequency in chronic calcular cholecystitis patients, to find out their antibiotic resistance pattern, and also to examine biofilm on the surface of gallstones and detect its correlation to antibiotic susceptibility pattern. Materials and methods: The study group included 257 patients with chronic calcular cholecystitis. S. typhi was isolated from gall bladder specimens. Antibiotics susceptibility tests of the isolates were done. Biofilm on surface of gallstones were visualized using scanning electron microscopy. Virulence (Vi) antibodies against S. typhi were studied in sera of 257 healthy controls. Results: 10.9% of patients were chronic typhoid carriers compared to only 3.5% of the controls (OR= 3.37, p=0.002). Multidrug resistance was detected in 35.7% of isolates. S. typhi biofilms on the surface of the 21/28 (75%) gallstones were detected. Ninety percent of the strains that produced maximum amount of biofilm were multidrug-resistant. Conclusion: Chronic calcular cholecystitis patients are more prone to typhoid carriage due to biofilm formation of S. typhi on the surface of gallstones

Role of gallstones in typhoid carriage in egyptian patients

Amaç: Salmonella typhi safra taşlarının üzerinde biyofilm oluşturarak kolonizasyonu ve taşıyıcılığı kolaylaştırır. Bu çalışmanın amacı, kronik taşlı kolesistiti olan hastalarda sıklığını saptamak, antibiyotik direnç paternlerini belirlemek, ayrıca safra taşları üzerindeki biyofilmi incelemek ve antibiyotik duyarlılık paterni ile ilişkisini araştırmaktır. Gereç ve yöntem: Çalışmaya kronik taşlı kolesistiti olan 257 hasta alındı. S. typhi safra kesesi örneklerinden izole edildi. İzolatların antibiyotik duyarlılıkları çalışıldı. Safra taşları üzerindeki biyofilm, tarama electron mikroskobu ile gözlendi. S. typhi‘ye karşı virulans (Vi) antikorları, 257 sağlıklı kontrolde çalışıldı. Bulgular: Hastaların %10,9’u, kontrollerin ise %3,5’i of kronik tifo taşıyıcısı idi (OR= 3,37, p=0,002). Çoklu ilaca direnç, izolatların %35,7’sinde saptandı. S. typhi biyofilmi, 28 safra taşının 21’inde (%75) saptandı. Aşırı miktarda biyofilm üreten kökenlerin %90’ı, çoklu ilaca dirençli idi. Sonuç: Kronik taşlı kolesistiti olan hastalar, S. typhi‘nin safra taşı üzerinde biyofilm yapmasına bağlı olarak tifo taşıyıcılığına eğilimlidirler.
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Journal of Microbiology and Infectious Diseases-Cover
  • ISSN: 2146-3158
  • Başlangıç: 2011
  • Yayıncı: Sağlık Araştırmaları Derneği
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Mısırlı hastalarda tifo taşıyıcılığında safra taşlarının rolü

Merwa Abdelazim MANSOUR, Tarik I. ZAHER, Amany M. IBRAHIM, Amira S. AHMED, Islam M. IBRAHIM, Ashraf GODA, Nehad F. AHMED