İ Wang, Yingna Song, Liangkun Ma, JUNTAO LİU, YİNGCHUN XU, JİE Yİ

Group B streptococcus detection in China: comparison of different screening methods and different sampling sites

Objectives: To evaluate the use of real-time polymerase chain reaction (RT-PCR) and bacterial culture methods to detect group B streptococcus (GBS) in Chinese pregnant women in the third trimester; to separately assess the prevalence of rectal and vaginal GBS colonization ; and to determine the antimicrobial susceptibility pattern of the isolates. Methodology: Samples were collected from 505 women at 35 and 37 weeks gestation at the Peking Union Medical College Hospital. Bacterial culture and RT-PCR were performed. Antimicrobial susceptibility to commonly used antibiotics was also analyzed. Results: The overall GBS colonization rate was 7.5%. The colonization rate, sensitivity, and negative predictive value of the bacterial culture method were 2.8%, 36.8%, and 95.1%, respectively, and these values were 7.3%, 97.4%, and 99.8%, respectively, for PCR (p<0.001). The GBS colonization rate of the rectum (6.7%) was higher than that of the vagina (2.8%) (p=0.005). Antimicrobial susceptibility testing showed that 100% were sensitive to penicillin, cephalosporin and vancomycin. Conclusions: RT-PCR was found to be a rapid and sensitive test for the detection of GBS colonization in Chinese pregnant women. Rectal swabbing was also important for detecting GBS colonization. β-lactams are the first-line antibiotics used for the treatment of GBS. J Microbiol Infect Dis 2016;6(4): 179-183 Group B streptococcus, Real-time polymerase chain reaction, Sampling sites,

Keywords:

Group B streptococcus, Real-time polymerase chain reaction, Sampling sites,

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1. Boyer KM, Gotoff SP. Strategies for chemoprophylaxis of GBS early-onset infections. Antibiot Chemother 1985; 35: 267- 380.
2. Jordan JA, Hall G, Davis T. Multicenter study evaluating performance of the smart Group B Streptococcus (GBS) assay using an enrichment protocol for detecting GBS colonization in patients in the antepartum period. J Clin Microbiol 2010; 48: 3193-3197.
3. Verani JR, McGee L, Schrag SJ. Prevention of perinatal group B streptococcal disease--revised guidelines from CDC. MMWR Recomm Rep 2010; 59: 1-36.
4. Jones N, Oliver K, Jones Y, etal. Carriage of group B streptococcus in pregnant women from Oxford, UK. J Clin Pathol 2006; 59: 363-366.
5. Kunze M, Ziegler A, Fluegge K, etal. Colonization, serotypes and transmission rates of group B streptococci in pregnant women and their infants born at a single University Center in Germany. J Perinat Med 2011; 39: 417-422.
6. Mereghetti L, Lanotte P, Rochoux A, et al. Application of the French guidelines for preventing neonatal group B streptococcal disease in a university hospital. Clin Microbiol Infect 2007; 13: 322-324.
7. Spaetgens R, DeBella K, Ma D, et al. Perinatal antibiotic usage and changes in colonization and resistance rates of group B streptococcus and other pathogens. Obstet Gynecol 2002; 100: 525-533.
8. Edwards RK, Novak-Weekley SM, Koty PP, et al. Rapid Group B streptococci screening using a Real-Time Polymerase Chain Reaction Assay. Obstet Gynecol 2008; 111: 1335- 1341.
9. de Tejada BM, Pfister RE, Renzi G, et al. Intrapartum Group B streptococcus detection by rapid polymerase chain reaction assay for the prevention of neonatal sepsis. Clin Microbiol Infect 2011; 17: 1786-1791.
10. Ke D, Menard C, Picard FJ, et al. Development of conventional and real-time PCR assays for the rapid detection of group B streptococci. Clinical chemistry 2000; 46: 324-331.
11. El Aila NA, Tency I, Claeys G, et al. Genotyping of Streptococcus agalactiae (group B streptococci) isolated from vaginal and rectal swabs of women at 35-37 weeks of pregnancy. BMC Infect Dis 2009; 9: 153.
12. Shen AD1, Zhang GR, Wang YH, et al. Susceptibility patterns and mechanisms of macrolide resistance in group B strep- tococcus isolates. Zhonghua Er Ke Za Zhi 2005 ;43(9):661- 664.
13. Wei CF1, She BC, Liang HS, et al. Prenatal Group B streptococcus test using real-time polymerase chain reaction. Taiwan J Obstet Gynecol 2009; 48 (2):116-119.
14. Dagnew AF, Cunnington MC, Dube Q, et al. Variation in reported neonatal group B streptococcal disease incidence in developing countries. Clin Infect Dis 2012; 55: 91-102.
15. Lu B, Li D, Cui Y, etal. Epidemiology of Group B streptococcus isolated from pregnant women in Beijing, China. Clinical microbiology and infection: the official publication of the European Society of Clinical Microbiology and Infectious Diseases 2014; 20: 370-373.
16. Binghuai L, Yanli S, Shuchen Z, etal. Use of MALDI-TOF mass spectrometry for rapid identification of group B Streptococcus on chromID Strepto B agar. Int J Infec Dis 2014; 27: 44-48.
17. Mitima KT, Ntamako S, Birindwa AM, etal. Prevalence of colonization by Streptococcus agalactiae among pregnant women in Bukavu, Democratic Republic of the Congo. J Infect Dev Ctries 2014; 8: 1195-1200.
18. Castellano-Filho DS, da Silva VL, Nascimento TC, etal. Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns. Braz J Microbiol 2010; 41: 1047-1055.
19. Bourgeois-Nicolaos N, Cordier AG, Guillet-Caruba C, etal. Evaluation of the Cepheid Xpert GBS Assay for Rapid Detection of Group B Streptococci in Amniotic Fluids from Pregnant Women with Premature Rupture of Membranes. J Clin Microbiol 2013; 51: 1305-1306.
20. Abdelazim IA. Intrapartum polymerase chain reaction for detection of group B streptococcus colonisation. Aust N Z J Obstet Gynaecol 2013; 53: 236-242.
21. Meyn LA, Krohn MA, Hillier SL. Rectal colonization by group B Streptococcus as a predictor of vaginal colonization. Am J Obstet Gynecol 2009; 201:71-77. 20. El Aila NA, Tency I, Claeys G, et al. Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women. BMC Infect Dis 2010; 10: 285.
22. Platt MW, McLaughlin JC, Gilson GJ, etal. Increased recovery of group B streptococcus by the inclusion of rectal bacterial culture and enrichment. Diagn Microbiol Infect Dis 1995; 21: 65-68.
23. Janapatla RP, Ho YR, Yan JJ, etal. The prevalence of erythromycin resistance in group B streptococcal isolates at a University Hospital in Taiwan. Microb Drug Resist 2008; 14: 293-297.
24. Capanna F, Emonet SP, Cherkaoui A, etal. Antibiotic resistance patterns among group B Streptococcus isolates: implications for antibiotic prophylaxis for early-onset neonatal sepsis. Swiss Med Wkly 2013; 143: 13778.
25. Clifford V, Heffernan HM, Grimwood K, etal. Variation in erythromycin and clindamycin resistance patterns between New Zealand and Australian group B streptococcus isolates. Aust N Z J Obstet Gynaecol 2011; 51: 328-332.
26. Bergseng H, Rygg M, Bevanger L, et al. Invasive group B streptococcus (GBS) disease in Norway 1996-2006. Eur J Clin Microbiol Infect Dis 2008; 27: 1193-1199.

Journal of Microbiology and Infectious Diseases-Cover

ISSN: 2146-3158
Başlangıç: 2011
Yayıncı: Sağlık Araştırmaları Derneği

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