Deneysel çalışma: yağ grefti sağ kalımında folik asit, vitamin C ve vitamin E etkilerinin araştırılması

Öz Bu çalışma, folik asit (vitamin B9), askorbik asit (vitamin C) ve α-tocopherol (vitamin E)'nin yağ grefti sağ kalımını artırmadaki potansiyel etkilerini araştırmayı amaçlamaktadır. Gereç ve Yöntem Sprague-Dawley sıçanlarının (n=10) sırt bölgeleri kasık yağı donörü alıcı bölgeleri olarak dört kadrana bölündü. İnkübasyondan sonra yağ greftleri bölgelere (%0,9 NaCl-sol kranyal, B9 vitamini-sağ kranyal, C vitamini-sol kaudal ve E vitamini-sağ kaudal) yerleştirildi. Üçüncü ayın sonunda yağ greftleri toplandı. Yağ greftleri canlılığını ortaya çıkarmak için perilipin ile immünohistokimyasal olarak değerlendirildi. Sonuçlar Folik asit, C vitamini ve E vitamininin perilipin ile yağ grefti sağkalım oranını iyileştirdiği gösterilmiştir (sırasıyla p<0.001, p<0.001, p<0.001). Sonuç Folik asidin (B9 vitamini) anjiyogenezi ve kollajen sentezini teşvik etmedeki etkilerinin, C vitamininin antioksidan özelliklerinin ve kollajen sentezine katılımının, E vitamininin güçlü antioksidan kapasitesinin, greft olarak kullanılan yağ dokusunu oksidatif stres ve inflamasyondan koruduğu, greftin vaskülarizasyonunu ve entegrasyonunu kolaylaştırdığı gösterilmiş, sağ kalımın artmasına yol açtığı görülmüştür. Ucuz ve temini ve uygulaması kolay bir ajan olan B9, C ve E vitaminlerinin, çalışmamızda immünohistokimyasal olarak yağ grefti sağ kalımını arttırdığı bulunmuştur. Bu ilaçlarla artan greft canlılığı gelecekteki klinik çalışmalara ve uygulamalara ilham verebilir.

Anahtar Kelimeler:

adipogenesis, ascorbic acid, fat graft survival, folic acid, vitamin C, vitamin E

Experimental study: effects of folic acid, vitamin C, and vitamin E on fat graft survival

Aim: This study explores the potential of folic acid (vitamin B9), ascorbic acid (vitamin C), and α-tocopherol (vitamin E) to enhance fat graft survival. Material and Method: The dorsal regions of the Sprague-Dawley rats (n=10) were divided into four quadrants as inguinal fat donor recipient sites. After incubation, fat grafts were placed into sites (0.9% NaCl-left cranial, vitamin B9-right cranial, vitamin C-left caudal, and vitamin E-right caudal). At the end of the third month, fat grafts were harvested. Fat grafts were evaluated immunohistochemically with perilipin to reveal viability. Results: Folic acid, vitamin C, and vitamin E were shown improved fat graft survival rate with perilipin (p<0.001, p<0.001, p<0.001, respectively). Conclusion: Effects of folic acid (vitamin B9) in promoting angiogenesis and collagen synthesis, vitamin C's antioxidant properties and involvement in collagen synthesis, vitamin E’s potent antioxidant capacity, appeared to protect grafted adipose tissue from oxidative stress and inflammation, facilitates graft vascularization and integration, leading to enhanced survival. As an inexpensive and easy-to-supply and apply agents, vitamins B9, C, and E were found to improve fat graft survival immunohistochemically in our study. Increased graft viability by these medicines may inspire future clinical studies and practice.

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