Ufkay KARABAY, R. Buğra HÜSEMOĞLU, Mehtap YÜKSEL EĞRİLMEZ, Hasan HAVITÇIOĞLU

Dermal Doku Mühendisliği Uygulaması için 3B Baskılı Doku İskelesi: In vitro Fibroblast Proliferasyonu

Fibroblastlar ekstrasellüler matriks üreten mezenkimal hücrelerdir. Fibroblastlar deride yara iyileşme sürecinde ve deri biyomühendisliğinde önemli bir role sahiptir. Bu çalışmanın amacı 3B baskılı Polilaktik asit (PLA) iskelelerinin insan dermal fibroblastlar (HDF) üzerindeki biyouyumluluk ve toksisite etkilerinin değerlendirilmesidir. İskeleler PLA malzemesi ile özel tasarım birleştirmeli yığma modellemesi (BYM) 3B yazıcı kullanılarak hazırlanmıştır. Çalışma için iki farklı por boyutunda (%35 ve %40) PLA iskeleler üretilmiştir. HDF’ler PLA iskelelere farklı sayılarda ekilmiştir. 3B PLA iskelelere ekilen HDF’lerin hücre çoğalması çalışmanın 12. ve 18. gününde WST-1 kolorimetrik yöntemi ile ölçülmüştür. HDF morfolojisi ve adhezyon özellikleri taramalı elektron mikroskopisi (SEM) ile görselleştirilmiştir. En yüksek hücre yoğunluğu 4 x 104 hücre ekilen grupta ölçülmüştür. SEM görüntüleri HDF’lerin iskele yüzeylerine tutunduklarını ve fiber-arası boşlukları doldurduklarını göstermiştir. Sonuçlarımız 3B baskılı üretilen PLA iskelelerin HDF ekiminde kullanılabileceğini ve yara iyileşmesi ve kişiselleştirilmiş ilaç denemelerinde potansiyel bir uygulamaya aday teşkil ettiklerini göstermiştir.

Anahtar Kelimeler:

PLA Doku İskelesi, Deri Doku Mühendisliği

3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro

Dermal fibroblasts are mesenchymal cells that produce extracellular matrix. Fibroblasts play an important role in the skin wound healing process and skin bioengineering. The aim of this study is to evaluate the behaviour of 3D printed polylactic acid (PLA) scaffolds in terms of biocompatibility and toxicity on human dermal fibroblasts (HDFs). Scaffolds were prepared with the PLA filament using a custom made fused deposition modeling (FDM) printer. We fabricated scaffolds with two different pore sizes (35% and 40%). HDFs were seeded at different densities on PLA scaffolds. The cell growth was measured by WST-1 colorimetric assay after 12 and 18 days of seeding HDFs on 3D PLA scaffolds. The morphology and the adhesion property of HDFs were visualized by scanning electron microscopy (SEM). HDFs showed a significant cell proliferation in 3D printd PLA scaffolds. The cell proliferation was highest at a density of 4 x 104 cells per well. SEM images showed that HDFs attached the surfaces of the scaffolds and filled the inter-fiber gaps. Our results showed that PLA scaffolds fabricated by 3D bioprinting is a promising candidate for HDF seeding and could have a potential application wound healing or personalized drug trials.

Keywords:

PLA Scaffolds, Skin tissue engineering,

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