Zübeyde HANOL BEKTAŞ, Füsun B UÇAR, Soner SAVAŞER

Isolation and Identification of Streptococcus parauberis From Freshwater Fish in Turkey

Streptococcus parauberis alfa hemolitik, gram pozitif kokoid bir bakteri olup Streptococcaceae familyasına aittir. Bu bakteri kültür balıklarında yoğun üretimden kaynaklı çok önemli bir hastalık olan streptokokozise neden olmaktadır ve balık çiftliği endüstrisinde önemli bir ekonomik kayba neden olmaktadır. Çalışmamızda Eğirdir gölü balıklarından (Sander lucioperca, Carassius gibelio, Cyprinus carpio) ve kültür balığı türü olan (Oncorhynchus mykiss, Walbaum)'dan 37 tane laktik asit bakterisi izole edilmiştir. Laktik asit bakterilerinin tanımlanması için MRS, TSA ve M17 agardan izole edilen 37 izolatın fenotipik ve morfolojik karakterleri koloni morfolojisi, hücre morfolojisi, hareketlilik, gram boyama, katalaz ve oksidaz üretimiyle yapılmıştır. Diğer biyokimyasal özellikler de Bergey's Manual of Systematic Bacteriology'e göre yapılmıştır. İzole edilen bakterilerinden 25 tanesi S. parauberis, 2 tanesi Vagococcus sp., 2 tanesi Lactococcus garvieae ve 5 tanesi de Lactococcus lactis olarak biyokimyasal yöntemler ve 16Sr RNA gen sekansıyla tanımlanmıştır fakat 3 tanesi (F65, F49 ve F50) tanımlanamamıştır. Çalışmamızda S. parauberis'in izolasyon ve identifikasyonu ilk olarak Türkiye'deki Eğirdir gölü balıklarından ve gökkuşağı alabalığından gerçekleştirildi

Streptococcus parauberis’in Türkiye’deki Tatlısu Balıklarından İzolasyon ve İdentifikasyonu

Streptococcus parauberis is an alpha-hemolytic gram positive coccoid bacterium belonging to the Streptococcaceae family. This bacterium cause streptococcosis is a major disease in cultured fish due to the intensification of aquaculture and causes significant economic losses in fish farm industry. In our study, we isolated a total of 37 lactic acid bacteria from wild fish (Sander lucioperca, Carassius gibelio, Cyprinus carpio) of Lake Eğirdir and cultured fish (Oncorhynchus mykiss, Walbaum) of farm in Turkey. For the isolation of bacteria phenotypic and biochemical characteristics of the 37 isolates obtained from the colonies grown on MRS, TSA and M17 agar characterized by determining colony morphology, cell morphology, motility, gram staining and the production of cytochrome oxidase and catalase. Further biochemical characteristics were determined using conventional tests according to Bergey’s Manual of Systematic Bacteriology. From the isolated lactic acid bacteria 25 of were identified as S. parauberis, 2 of as Vagococcus sp., 2 of as Lactococcus garvieae and 5 of as Lactococcus lactis by culture-based, biochemical test and 16Sr RNA gene sequencing techniques but 3 of them couldn’t (F65, F49 and F50) identified. In this study, the main objective of us is to identify S. parauberis with conventional and 16Sr RNA gene sequencing techniques from wild fish in Lake Eğirdir and in cultured fish in fish farm of Egirdir-Turkey

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