In this study, it was aimed to investigate the antibodies of Brucellosis in dogs that were individuals bringing to Balikesir University Veterinary Faculty Animal Hospital. Blood samples were taken from dogs (n=90) for evaluating the Brucella melitensis, abortus, and suis antibody positivity by using the rapid test kits (Brucella Ab test kit, Bionote, Inc. Korea). A drop of serum sample and four drops of test solution were dropped into the sample well of the test kits and, then after the 20 minutes, it was decided whether Brucella positive or not. According to the tests, all animals were determined as Brucella antibody negative. In conclusion, this study demonstrated that dogs do not have Brucella antibodies. We suggested that screening for brucellosis in dogs should be supported by other diagnostic methods [Enzyme-Linked ImmunoSorbent Assay (ELISA), Rose bengal, Serum Agglutination Test (SAT), Commbs Test], for detecting the Brucella canis antibodies.