Comparative study for production and charcterization of D-Xylanase produced from locally isolate Aspergillus niger by submerged and solid state fermentation

Xylanase was pooled from Aspergillus niger that was cultivated on czepak dox with addition rice husk. Condition affecting xylanase production were assessed these included p H, temperature of cultivation, carbon source, nitrogen source, incubation period, and inoculums size for both types of culture. Results obtained were as follow ion exchange DEAD-Cellulose specific activity from solid state fermentation (І, ІІ) were (902.43,3666.6 U/mg), with yield (0.602,0.212%) and No. of fold (1.24,5.034). The specific activity from submerged fermentation (P 1, P2) were (1861.76,910 U/mg), with yield (1.033, 0.531%) and No. of fold (5.570, 2.790). the optimum and stability p H for enzyme activity type (ІІ) produced by solid state fermentation (7.0 and 7.0) respectively. the optimum and stability temperature for enzyme type (ІІ) produced by solid state fermentation 50°C and 70 °C respectively. and the optimum and stability p H for enzyme activity type (P1) produced by submerged (7.0 and 7.0) respectively. the optimum and stability temperature for enzyme activity type (P1) produced by submerged fermentation 30 and 60 °C respectively.

Keywords:

xylanase, solid state fermentation, purification submerged fermentatio,

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Journal of International Environmental Application and Science-Cover

ISSN: 1307-0428
Yayın Aralığı: Yılda 4 Sayı
Başlangıç: 2006
Yayıncı: Selçuk Üniversitesi

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