Medicinal plants are a natural source that possesses significant biological functions. Cistus species which are medical plant are of antibacterial, antioxidant and cytotoxic activities. This study was perfpormed to investigate the antibacterial, antioxidant and DNA interaction properties of Cistus creticus extracts. Samples of Cistus creticus L. were obtained in May 2018 in the region of Amasya. The different extract of Cistus criticus leaves was obtained by using soxhlet apparatus for 4 hours. Antibacterial activity of extracts was tested against four Gram positive and four Gram negative by the disc diffusion method. Antioxidant activities of extracts were determined with DPPH radical scavenging, ferric reducing and metal chelating methods. The ability to repair the plasmid DNA breaks created by hydroxyl radicals was also determine using pUC18 plasmid DNA.  As a result, Cistus creticus. extracts has strong inhibitory activity against all tested bacteria. The chloroform extract was also very effective against Gram positive bacteria especially Bacillus cereus. Among the Gram negative bacteria, the most susceptible bacterium was identified as Pseudomonas aeruginosa. Moreover, ethanol extracts had repair effects on plasmid DNA in H2O2 condition. Among the different extracts of Cistus creticus chloroform extract showed the highest in vitro antioxidant activity. In this study, Cistus creticus collected from Amasya has shown significant antibacterial, antioxidant and DNA interaction activity and could be therefore a useful source in the discovery of new antibacterial and antioxidant compounds. However, the use of extracts as a complement to the treatment requires further research to thoroughly understand the activities and interactions with recommended medicines.