Sami KINIKLI, Esra KAYA KILIÇ, Kader ARSLAN, Salih CESUR, Çiğdem ATAMAN HATİPOĞLU, Serap YAĞCI, Bedia DİNÇ

Candida suşlarının tanımlanmasında CHROMagar Candida besiyerinin performansının değerlendirilmesi

Amaç: Candida enfeksiyonlarının tedavisi Candida türlerine göre farklılık göstermektedir. Rutin ve klasik yöntemlerle identifikasyon birkaç günü alabilmektedir. Bu nedenle, etkili antifungal tedavinin başlanabilmesi için Candida türlerinin hızlı tanısı önemlidir. Gereç ve Yöntem: Çalışmaya dahil edilen 61 Candida suşu CHROMagar Candida besiyerine ekilip üreticinin önerileri doğrultusunda uygun şartlarda inkübe edildikten sonra, 24. ve 48. saatlerde değerlendirildi.Bulgular: Çalışmada CHROMagar Candida besiyeri ile referans yöntemler karşılaştırıldığında, CHROMagar Candida ile 61 örneğin 36’sında (%40,9) referans yöntemle aynı tür tayini saptanmıştır. Yirmi beş örnekte (%59,1) ise CHROMagar Candida besiyerinde Candida türleri üremiş ancak tür tayini yapılamamıştır. Sonuç: CHROMagar Candida besiyeri germ tüp testine göre Candida albicans‘ın yanısıra, Candida parapsilosis türlerinin ayrımına imkan sağlamasına rağmen, diğer Candida türlerin ayrımına imkan sağlamaması, farklı renkler nedeniyle sorunlar yaşanması ve maliyetinin yüksek olması dezavantajlarıdır.

Anahtar Kelimeler:

Candida türleri, hızlı tanı, kromojenik besiyeri

Assessment of the performance of CHROMagar Candida media in identification of candida strains

Objective: The treatment of Candida infections differs according to Candida species. The identification by routine and conventional methods can take several days. For this reason, rapid diagnosis of Candida species is important for initiating an effective antifungal therapy. Material and Method: Sixty one Candida strains included in the study were evaluated at 24th and 48th hours after they were cultivated on CHROMagar Candida medium and incubated under appropriate conditions in accordance with the manufacturer's recommendations.Results: When CHROMagar Candida medium and reference methods were compared in the study, it was found that CHROMagar Candida and referance method were detected the same species in 36 of the 61 samples (40.9%). Candida species were produced in CHROMagar Candida medium in 25 samples (59.1%) but the identification of species could not be made. Conclusion: The CHROMagar Candida medium allows the discrimination of Candida parapsilosis species as well as Candida albicans according to the germe tube test, but does not allow the discrimination of other Candida species. It also has disadvantages of having problems due to different colors and high cost.

Keywords:

Candida species, rapid diagnosis, chromogenic medium,

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Journal of Health Sciences and Medicine-Cover

Yayın Aralığı: Yılda 6 Sayı
Başlangıç: 2018
Yayıncı: MediHealth Academy Yayıncılık

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