Hydrogen peroxide-induced oxidative stress and apoptosis in SH-SY5Y cells: Protective effect of Momordica charantia fruit extract

Oxidative stress triggers apoptosis in neuronal cells, resulting in cellular loss, which is critical in the pathogenesis of neurodegenerative diseases. Momordica charantia (MC), a traditional medicinal plant, is famous for its variety of health benefits, including its anti-diabetic, anti-inflammatory, and antioxidant properties. The purpose of this study was to investigate on how MC might affect oxidative stress and apoptosis caused by H2O2. First, we investigated whether ethanol extract of MC in the presence of H2O2 attenuated cell death in human neuroblastoma SH-SY5Y cells. MC improved H2O2-stimulated intracellular reactive oxygen species (ROS) production. Additionally, MC reduced caspase activation, which greatly improved cell viability and avoided H2O2-induced apoptosis. Through its anti-oxidant and anti-apoptotic properties, MC ethanol extract has been shown to be effective in protecting against H2O2-induced cell death.

Keywords:

Hydrogen peroxide Oxidative stress, Apoptosis,

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1. Chen X, Guo C, Kong J. Oxidative stress in neurodegenerative diseases. Neural Regeneration Research 2012;7(5):376-85.
2. Solanki I, Parihar P, Parihar MS. Neurodegenerative diseases: From available treatments to prospective herbal therapy. Neurochemistry International 2016;95:100-8.
3. Rahman MH, Bajgai J, Fadriquela A, Sharma S, Trinh TT, Akter R, Jeong YJ, Goh SH, Kim CS, Lee KJ. Therapeutic Potential of Natural Products in Treating Neurodegenerative Disorders and Their Future Prospects and Challenges. Molecules. 2021;26(17):5327.
4. Ataie A, Shadifar M, Ataee R. Polyphenolic Antioxidants and Neuronal Regeneration. Basic Clin Neurosci. 2016;7(2):81-90.
5. González-Sarrías A, Núñez-Sánchez MÁ, Tomás-Barberán FA, Espín JC. Neuroprotective Effects of Bioavailable Polyphenol-Derived Metabolites against Oxidative Stress-Induced Cytotoxicity in Human Neuroblastoma SH-SY5Y Cells. J Agric Food Chem. 2017;65(4):752-758.
6. Kinarivala N, Patel R, Boustany RM, Al-Ahmad A, Trippier PC. Discovery of Aromatic Carbamates that Confer Neuroprotective Activity by Enhancing Autophagy and Inducing the Anti-Apoptotic Protein B-Cell Lymphoma 2 (Bcl-2). J Med Chem. 2017;60(23):9739-9756.
7. Yan L, Guo MS, Zhang Y, Yu L, Wu JM, Tang Y, Ai W, Zhu FD, Law BY, Chen Q, Yu CL, Wong VK, Li H, Li M, Zhou XG, Qin DL, Wu AG. Dietary Plant Polyphenols as the Potential Drugs in Neurodegenerative Diseases: Current Evidence, Advances, and Opportunities. Oxid Med Cell Longev. 2022;2022:5288698.
8. Kubola J, Siriamornpun S. Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chem. 2008;110(4):881-90.
9. Jia S, Shen M, Zhang F, Xie J. Recent Advances in Momordica charantia: Functional Components and Biological Activities. Int J Mol Sci. 2017;18(12):2555.
10. Ramalhete C, Gonçalves BMF, Barbosa F, Duarte N, Ferreira MU. Momordica balsamina: phytochemistry and pharmacological potential of a gifted species. Phytochem Rev. 2022;21(2):617-646.
11. Aljohi A, Matou-Nasri S, Ahmed N. Antiglycation and Antioxidant Properties of Momordica charantia. PLoS One. 2016;11(8):e0159985.
12. Dandawate PR, Subramaniam D, Padhye SB, Anant S. Bitter melon: a panacea for inflammation and cancer. Chin J Nat Med. 2016;14(2):81-100.
13. Oyelere SF, Ajayi OH, Ayoade TE, Santana Pereira GB, Dayo Owoyemi BC, Ilesanmi AO, Akinyemi OA. A detailed review on the phytochemical profiles and anti-diabetic mechanisms of Momordicacharantia. Heliyon. 2022;8(4):e09253.
14. Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harb Protoc. 2018;2018(6).
15. Ng NS, Ooi L. A Simple Microplate Assay for Reactive Oxygen Species Generation and Rapid Cellular Protein Normalization. Bio Protoc. 2021;11(1):e3877.
16. Pillai-Kastoori L, Schutz-Geschwender AR, Harford JA. A systematic approach to quantitative Western blot analysis. Anal Biochem. 2020;593:113608.
17. Cumaoğlu A, Rackova L, Stefek M, Kartal M, Maechler P, Karasu C. Effects of olive leaf polyphenols against H₂O₂ toxicity in insulin secreting β-cells. Acta Biochim Pol. 2011;58(1):45-50.
18. Smith JA, Park S, Krause JS, Banik NL. Oxidative stress, DNA damage, and the telomeric complex as therapeutic targets in acute neurodegeneration. Neurochem Int. 2013;62(5):764-75.
19. Zhao RZ, Jiang S, Zhang L, Yu ZB. Mitochondrial electron transport chain, ROS generation and uncoupling (Review). Int J Mol Med. 2019;44(1):3-15.
20. Park J, Lee J, Choi C. Mitochondrial network determines intracellular ROS dynamics and sensitivity to oxidative stress through switching inter-mitochondrial messengers. PLoS One. 2011;6(8):e23211.
21. Kim R, Emi M, Tanabe K, Murakami S, Uchida Y, Arihiro K. Regulation and interplay of apoptotic and non-apoptotic cell death. J Pathol. 2006;208(3):319-26.
22. Kim KB, Lee S, Kang I, Kim JH. Momordica charantia Ethanol Extract Attenuates H₂O₂-Induced Cell Death by Its Antioxidant and Anti-Apoptotic Properties in Human Neuroblastoma SK-N-MC Cells. Nutrients. 2018;10(10):1368.
23. Thamaraikani T, Chitra V, Chitra K. In vitro neuroprotective effect of charantin from Momordica charantia against neurotoxin and endoplasmic reticulum stress-induced cell death in SH-SY5Y cells. International Journal of Green Pharmacy. 2018;12(3):1-6.
24. Pattarachotanant N, Prasansuklab A, Tencomnao T. Momordica charantia L. Extract Protects Hippocampal Neuronal Cells against PAHs-Induced Neurotoxicity: Possible Active Constituents Include Stigmasterol and Vitamin E. Nutrients. 2021;13(7):2368.
25. Malik ZA, Singh M, Sharma PL. Neuroprotective effect of Momordica charantia in global cerebral ischemia and reperfusion induced neuronal damage in diabetic mice. J Ethnopharmacol. 2011;133(2):729-34.
26. Cai H, Huang LY, Hong R, Song JX, Guo XJ, Zhou W, Hu ZL, Wang W, Wang YL, Shen JG, Qi SH. Momordica charantia Exosome-Like Nanoparticles Exert Neuroprotective Effects Against Ischemic Brain Injury via Inhibiting Matrix Metalloproteinase 9 and Activating the AKT/GSK3β Signaling Pathway. Front Pharmacol. 2022;13:908830.