Kazım BOZDEMİR, Bülent ULUSOY, Arife SEZGİN, Ahmet AKKOZ, Özcan EREL, Mehmet Hakan KORKMAZ

Komplikasyonsuz Kronik Otitis Media’da Thiol/Disülfit Dengesi

Giriş: Kronik mukozal inflamatuar bir hastalık olan kronik otitis media patogenezinde oksidatif stresin rol oynayabileceğini düşündük. Bu amaçla yeni bir oksidatif stres belirteci ile bu durumu araştırmayı amaçladık.Gereç ve yöntemler: Kronik ototis media tanısı konulan 30 hasta ve sağlıklı gönüllülerden oluşan 30 birey kontrol grubu olarak çalışmaya dahil edildi. Kan örnekleri hastaneye yatışta ameliyat öncesinde alındı. Kontrol grubundaki kan örnekleri polikliniğimize başvuru esnasında alındı. Thiol/disülfit düzeyleri Erel ve Neşelioğlu tarafından geliştirilen yeni bir yöntemle analiz edildi.Sonuçlar: Kronik otitis media grubuna 30 birey (20 kadın, 10 erkek) dahil edildi. Konrol grubu olarak da 30 birey (8 kadın, 22 erkek) alındı. Gruplar içerindeki cinsiyet dağılımındaki farklılık istatistiksel olarak anlamlı (p=0.004) iken yaş dağılımı açısından anlamlı farklılık yoktu(p=0.072). Ölçülen native thiol disülfit ve total thiol değerleri açısından gruplar arasında anlamlı farlılık yoktu. Sonuç:Bu çalışma, kronik otitis medialı hastalarda thiol oksidasyonunun bir sonucu olarak dinamik thiol disülfit dengesi disülfit formasyonuna doğru kaydığını göstermesine rğmen gruplar arasında anlamlı bir farklılık bulunmadı.

Anahtar Kelimeler:

Oxidative stress, Thiol/disulphide homeostazisi, kronik otitis media

Thiol-disulphide homeostasis in noncomplicated chronic otitis media

Introduction: We hypothesized that oxidative stress plays a role in the pathogenesis of chronic otitis media, chronic mucosal inflammatory disease. We aimed to investigate a novel oxidative stress marker in this study. Materıals and methods: Thirty patient with chronic otitis media as the patient group and 30 healthy volunteer subjects as the control group were admitted to the study. Blood samples were taken when they admitted to our clinic before surgical intervention. In healthy volunteers, blood samples were taken when they were admitted to our policlinic. Thiol/disulphide levels were analyzed with a newly developed method by Erel and Neselioglu.Results: 30 subjects were included in chronic otitis media group (20 females, 10 males). 30 subjects were included in the control group (8 females and 22 males). Sex distribution within the groups was significantly different (p=0.004). There was no significant difference between groups with respect to the age distribution (p=0.072). Measured native thiol, disulphide and total thiol values of the groups were not significantly different from each other. Conclusion: This study demonstrated that although dynamic thiol/disulphide homeostasis was shifted towards disulphide formation as a result of thiol oxidation in patients with chronic otitis media. But we could not find any significant difference between groups.

Keywords:

Oxidative stress, Thiol/disulphide homeostasis, chronic otitis media,

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