Oxidative and antioxidative responses in submandibular and parotis glands of rats exposed to long-term extremely low frequency magnetic field
Amaç: Bazı epidemiyolojik ve laboratuar çalışmalar oldukça düşük frekanslı manyetik alan ile kanser arasında ilişki olduğunu ileri sürmektedir. Ancak bu etkileşimin altında yatan mekanizma tam olarak bilinmemektedir. Çalışmamızın amacı ratlardaki submandibular ve parotis bezleri üzerindeki uzun süreli oldukça düşük frekanslı manyetik alanın oksidatif ve antioksidatif etkilerini araştırmaktır. Yöntemler: Ratlar; çalışma düzeneği olarak günde 2 saat, yedi gün ve 10 ay boyunca insanlar için güvenli kabul edilen 100 ve 500 µT\'lık manyetik alana maruz bırakıldı. Aynı prosedür şham konrol grubuna uygulandı, fakat manyetik alan jeneratörü kapalı tutuldu. Çalışmanın bittiği 10. ay\'dan sonra katalaz, malondilaldehit, myeloperoksidaz, total antioksidan kapasite, total oksidan status ve oksidatif stress indeks parametreleri ölçüldü. Bulgular: ELF-100 ve ELF-500 uygulama grubunda bulunan ratların submandibular dokusunun bazı oksidatif ve antioksidatif parametrelerinde değişiklik olmakla birlikte, bu değişiklikler istatistiksel olarak anlamlı değildi (p>0.05). Ancak hem ELF-100 ve hem de ELF-500 uygulama grubunda bulunan ratların parotis bezlerinin katalaz düzeyinde anlamlı azalma gözlendi (p
Anahtar Kelimeler:
Oldukça düşük frekanslı manyetik alan, Oksidatif stres, Antioksidan enzim, submandibular bez, Parotis bezi
Oxidative and antioxidative responses in submandibular and parotis glands of rats exposed to long-term extremely low frequency magnetic field
Background: Some epidemiologic and laboratory studies have suggested a possible associations between exposure to extremely low frequency magnetic field (ELF-MF) and cancer. However, it is not known underlying mechanisms of this interaction. The aim of the study was to investigate the possible oxidative damage induced by long-term ELF-MF exposure on submandibular and parotis glands of rats. Methods: Rats in the experimental group were exposed to 100 and 500 µT ELF-MF (2 h/day, 7 days/week, for 10 months) corresponding to exposure levels that are considered safe for humans. The same experimental procedures were applied to the sham group, but the ELF generator was turned off. The levels of catalase (CAT), malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidative capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were measured in rat submandibular and parotis gland. Results: Although some oxidative and antioxidative parameters of submandibular gland were altered by ELF-100 and ELF-500 exposure groups, these changes were not statistically significant ( p >0.05). However, a decrease observed in CAT levels of parotid gland in both the ELF-100 and ELF-500 exposure groups (p
Keywords:
Extremely Low-Frequency Magnetic Field, oxidative stress, antioxidant enzyme, submandibular gland, parotis gland,
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- Başlangıç: 2010
- Yayıncı: Sağlık Araştırmaları Derneği