Aggregates of α-synuclein in brain tissue homogenates measured by newly designed Multimer-PAGE techniques
Atypical aggregation of α-synuclein in the brain is linked to Parkinson’s disease (PD) progression. Mutimers of α-synuclein may be important for the biological function of α-synuclein. Currently, reliable methods to compare α-synuclein multimer abundance in biological samples are limited and sophisticated. A new technique termed “multimer-PAGE,” was designed by Killinger and Moszczynska, which combines in-gel chemical cross-linking with several common electrophoretic techniques to measure the multimerization of α-synuclein in complex biological samples of brain tissue lysates. Using this technique, it was possible to compare the ratio of α-synuclein multimers between brain tissue samples without the need for specialized equipment.
Keywords:
Brain, Multimer-PAGE techniques, Parkinson’s disease α-synuclein,
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- Sulaiman MA, Al-Farsi YM, Al-Khaduri MM, Waly MI, Saleh J, Al-Adawi S. Psychological burden among women with polycystic ovarian syndrome in Oman: a case-control study. Int J Womens Health. 2017;9:897-904.
- Yayın Aralığı: Yıllık
- Başlangıç: 2008
- Yayıncı: Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği