Abstract In this work the influence of oil amount in sediment was determined using various reference oils by UVF. 15 reference oils which were transported from the Black Sea used for plotting of standard curve. The differences of oil amount found through reference oil equations were varied in sediment as 17.0-28.1 % in wet weight, 13.4-14.1 % in oven, 12.2-26.2 in freeze dryer. These results showed that reference oils have an important role on the amount of oil in sediment. In the determination of oil pollution, the pollutant oil must be used in the analysis as references for each time.