Abstract The GOTIA ship accident occured on 06 Oct 2002 at Emirgan quay, in Bosphorus, 25 ton fuel was spilled into the sea. In this study the 279 samples of sea water, 6 mussel, 1 alg and GOTIA fuel oil were analysed by UVF and 287 samples by GC/MS and 200 samples by fingerprinting teclmique. The oil contamination was spread out at Istinye, northem part of Bosphorus at Golden Horn and at Yenikapi (near south end of Bosphorus). The maximum oil levels found after the accident were 813.5 mg/L in Bosphorus is 7.3 mg/L in Golden Horn and 27.4 mg/L at Yenikapi. The oil pollution area related to GOTIA fuel oil was proved by using fingerprinting technique.