Abdullah TASHTOUSH

7320

Patojenik Bakteri Algılama İçin Sarj Tabanlı Kapasitans Ölçümü Kullanarak Nano-Amplifikasyon Stratejisi

Amaç: Bu çalışma, patojenik hastalık gibi klinik ve sağlık uygulamalarında tespit seviyesini arttırmak ve prob pedlerinin sayısını azaltmak için MultiLab-On-Single-Chip (MLoC) sistemi mikro imalatı için bir nano-amplifi kasyon stratejisi sunmaktadır.Materyal veMetod:Uygulama malzemesi katkılı silikon, Şarj bazlı kapasitans ölçüm (CBCM) tekniği, 350 nm teknolojisi ile Tamamlayıcı Metal Oksit-Yarıiletken(CMOS) tekniği kullandı. Algılayıcı kapasitöre uygulanan, ancak referans olana uygulanmayan hedefl enen bakterilerin mevcudiyetindeoluşan geçirgenliğin sonucu olan, sistem kapasitansın varyasyonunu tespit etmek ve işlemek için interdijite kapasitör yapısı, yüke bağlıkapasitans ölçüm devresi kullanıldı.Bulgular: Sistem, literatür sonuçlarına göre düşük seviyede bakteriyel konsantrasyon için iyi bir duyarlılık ortaya koymuştur. Devre, 100 aF civarındakapasitans değişikliklerini ölçüyordu. Devrenin çıkışındaki gürültü 78.7µV civarındadır.Tartışma: CMOS biyosensör bazlı şarj bazlı kapasitans ölçüm sistemi, bakteriyel patojenlerin hücre tespiti için test edilmiş ve deneysel olarakdoğrulanmıştır. Fonksiyonel algılama kapasitansı, referans kapasitansı ve ara-yüz devresinin pasifl eştirilmesi de dahil olmak üzere bu türbiyosensör sistemlerinin üç önemli gerekliliği tartışılmıştır. Sistem seri olarak üretilebilen hızlı, düşük güç ve minyatür bir platform sağlar. 
Anahtar Kelimeler:

Escherichia coli, Biyosensörler, CBCM, Escherichia coli, sağlık uygulamalar, MLoC sistem, dielektrik sabiti

Nano-Amplification Strategy Using Charge-Based Capacitance Measurement for Pathogenic Bacteria Detection

Objective: This work will present a nano-amplifi cation strategy for microfabricating Multi-Lab-On-Single-Chip (MLoC) system to enhance the level ofdetection and also reduced the number of probe pads for clinical and health care applications, such as pathogenic disease.Material andMethod:The Charge-Based Capacitance Measurement (CBCM) technique used Complementary Metal-Oxide-Semiconductor (CMOS) with 350nm technology, the implementation material is doped silicon. The system employs an interdigitated capacitor structure, charge basedcapacitance measurement circuitry to detect and process the variation of the capacitance, which is a function of the permittivity in thepresence of targeted bacteria that is applied to the sensing capacitor but not to the reference one.Findings andResults:The system appeared good sensitivity for low level concentration of bacterial comparing to the literature results. The circuit was measuringthe capacitance changes around 100 aF. The noise at the output of the circuit is around 78.7µV.Conclusion: CMOS biosensor-based charge-based capacitance measurement system has been fabricated, testing and experimentally validated forbacterial pathogens cell detection. Three important requirements of such biosensor systems including functionalized sensing capacitance,passivating the reference capacitance and interface circuit were discussed. The system provides a rapid, low power, and miniaturizedplatform that can be mass-produced.
Keywords:

Biosensors, CBCM, Escherichia coli, health applications, MLoC system, permittivity,

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Journal of Biotechnology and Strategic Health Research-Cover
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 2017
  • Yayıncı: Deneysel, Biyoteknolojik, Klinik ve Stratejik Sağlık Araştırmaları Derneği
Arşiv
Sayıdaki Diğer Makaleler

Aile Hekimleri, Pediatristler ve Eczacıların Probiyotik Kullanımları Hakkında Bilgi, Tutum ve Davranışları

Mustafa ALTINDİŞ, Mustafa Baran İNCİ, Bahri ELMAS, Elif ÖZÖZEN ŞAHİN, Elmas Pınar KAHRAMAN, Rıdvan KARAGÖZ, Gökhan KÜÇÜKKARA, Selma ALTINDİŞ

Patojenik Bakteri Algılama İçin Sarj Tabanlı Kapasitans Ölçümü Kullanarak Nano-Amplifikasyon Stratejisi

Abdullah TASHTOUSH

ALTIN NANOPARTİKÜL İŞARETLİ BİYOSENSÖR ile E.COLİ’NİN HIZLI ve DUYARLI TESPİTİ

Sümeyra SAVAŞ

TAMAMLAYICI TIP VE GÜNCEL APİTERAPI UYGULAMALARI

Yıldıray Aydın, İbrahim Tekeoğlu