Gulsen YILDIZAY, Filiz GUNAL, Firdevs GURER, Hikmet HASSA, Atilla YILDIRIM

The effects of the presence of the hydrochlorofluorocarbon (HCFC)-123 containing fire extinguisher in laboratories upon sperm motility and embryo development

This study was conducted to investigate in vitro effects of HCFC-123 on sperms and embryos due to the possibility of gas leakage from fire extinguishers and in vivo studies reporting prenatal toxicity and implantation failure with some compounds of halogenated hydrocarbon family. This study was designed in two study groups; first investigating embryo toxicity in rat embryos and the second, studying possible spermatotoxic effects on human sperm with sperm survival test. The study was conducted in two experiment environments. One of the systems has the presence of HCFC-123 containing fire extinguisher and the second system is without fire extinguisher. As results, fertilization rates were similar in HCFC-123 containing fire extinguisher group and HCFC-123 free group (57%, 60% respectively). Cleavage rate was 45% in HCFC-123 containing fire extinguisher group and found as 50% in HCFC-123 free group (p>0.05). HCFC-123 containing fire extinguisher did not have harmful affects upon sperm motility. In conclusion, HCFC-123 containing fire extinguishers can be used safely in IVF laboratorie

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[1]. Molina MJ, Roland FS. 1974. Stratospheric sink for chlorofluoromethanes: Chlorine atom catalyzed destruction of ozone. Nature. 249: 810–812.
[2]. Emmen HH, Hoogendijk EMG, Klöpping-Ketelaars WAA, Muijser H, Duistermaat E, Ravensberg JC, Alexander DJ, Borkhataria D, Rusch GM, Schmit B. 2000. Human Safety and Pharmacokinetics of the CFC Alternative Propellants HFC 134a (1,1,1,2-Tetrafluoroethane) and HFC 227
(1,1,1,2,3,3,3-Heptafluoropropane) following whole-body exposure. Reg Toxicol Pharmacol. 32: 22-35.
[3]. Brown PC, Hayes LC, Huq F, Herlihy C, Picker K, Webster WS. 1998. In vitro assessment of effect of halogenated hydrocarbons: Chloroform, dichloromethane, and dibromoethane on embryonic development of rat. Teratology. 57: 321-333.
[4]. Schmidt R, Scheufler H, Bauer S, Wolff L, Pelzing M, Herzschuh R. 1987. Toxicological investigations in the semiconductor industry: III studies on prenatal toxicity caused by waste products aluminum plasma etching processes. Cell Biol Toxicol. 3: 391-406.
[5]. Available: http://www.afeas.org/paft/hcfc-123.html.
[6]. Dodd DE, Leahy HF, Feldmann ML, English JH, Vinega A. 1999. Reproductive toxicity screen of trifluoroiodomethane (CF (3) I) in Sprague-Dawley rats. Inhal Toxicol. 11: 1041-1055.
[7]. Rusch GM, Millischer RJ, de Rooij C, Brooker AJ, Hughes E, Coombs D. 1995. Inhalation teratology and two-generation reproduction studies with 1,1-Dichloro- 1-Fluoroethane (HCFC-141b). Food Chem Toxicol. 33:285-300.
[8]. Malinverno G, Rusch GM, Millischer RJ, Hughes EW, Schroeder RE, Coombs DW. 1996. Inhalation teratology and reproduction studies with 1,1-dichloro-2,2,2- trifluoroethane (HCFC-123). Fundam Appl Toxicol. 34:276-87.
[9]. Giesy JP, Kannan K, Jones PD 2001. Global Biomonitoring of Perfluorinated Organics. The Scientific World. 1: 627-629.
[10]. Wickramaratne GA, Tinston DJ, Kinsey DL, Doe JE. 1998. Assessment of reproductive toxicology of bromochlorodifluoromethane (BFC, halon 1211) in the rat. Br J Ind Med. 45:755-760.
[11]. Sass-Kortsak AM, Holness DL, Stopps GJ. 1985. An accidental discharge of a Halon 1301total flooding fire extinguishing system. Am Ind Hyg Assoc. J. 46: 670-673.
[12]. Kang KS, Park JE, Ryu DY, Lee YS. 2004. Effects and neuro-toxic mechanisms of 2,2‘, 4,4‘, 5,5‘- hexachlorobiphenyl and endosulfan in neuronal stem cells. Toxicology. 196: 77-86.
[13]. Boone WR, Johnson JE, Locke AJ, Crane MM. 4th, Price TM. 1999. Control of air quality in an assisted reproductive technology laboratory. Fertility Sterility. 71:150-154.