M. M. Abu TEIR, J. H. GHITHAN, S. M. DARWISH, M. M. ABU-HADID

113144

Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach

Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach

The interaction between progesterone and human serum albumin has been investigated. This interaction was studied by UV-absorption and fluorescence spectroscopy. From spectral analysis progesterone showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. The binding constant (K) is estimated 6.56×102 M-1 at 293 K. FT-IR spectroscopy with Fourier self-deconvolution technique was used to determine HSA secondary structure and progesterone binding mechanisms. The observed spectral changes indicate the formation of H-bonding between progesterone and HSA molecules which can be related to the intensity decrease in the absorption band of α-helix relative to that of β-sheets
Keywords:

progesterone, amide I-III , binding mode, binding constant protein secondary structure, Fourier transform IR, UV–spectroscopy, Flurosence spectroscopy,

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Journal of Applied Biological Sciences-Cover
  • ISSN: 1307-1130
  • Yayın Aralığı: 6
  • Başlangıç: 2007
  • Yayıncı: Nobel Bilim ve Araştırma Merkezi
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