Serum biochemical values were determined in clinically healty 12 adult (>12 month) female Gazella subgutturosa in Ceylanpınar, Şanlıurfa, Turkey. In the present study, we determined the concentrations of glucose (72.4 ± 24.9 mg/dL), total bilirubin (0.31 ± 0.1 mg/dL), blood urea nitrogen (15.6 ± 4.39 mg/dL), creatinine (0.9 ± 0.07 mg/dL), uric acid (0.7 ± 0.33mg/dL), total protein (7.16 ± 0.33 g/dL), albumin (3.27 ± 0.47 g/dL), cholesterol (52.2 ± 12.72 mg/dL), triglyceride (51.24 ± 17.05 mg/dL) sodium (152.1 ± 4.79 mEq/L), potassium (5.08 ± 0.39 mEq/L), calcium (9.1 ± 0.84 mg/dL), phosphorous (6.6 ± 0.54 mg/dL), chloride (110 ± 3.0 mEq/L), magnesium (1.98 ± 0.08 mg/dL). Furthermore the activity of enzymes AST (189.8 ± 103.7 IU/L), ALT (12.20 ± 9.60 IU/L), ALP (103.8 ± 119.2 IU/L), GGT (50.8 ± 52.5 IU/L), LDH (520.6 ± 179.8 IU/L), CK (194.6 ± 113.8 IU/L), amylase (75.2 ± 32.8 IU/L) were measured. In conclusion we determined reference biochemical values and health status’ of Gazella subgutturosa in Ceylanpınar, Şanlıurfa, Turkey