In this study, it was aimed to determine the resistance to root-knot nematodes and Verticillium wilt of 288 tomato (Lycopersicum esculentum Mill.) samples by DNA markers. First, it was established that whether the tomato samples have primer binding site or not by the CAPS primers which are specifically designed for both of the diseases. In the analysis of primer binding site to root-knot nematodes and Verticillium wilt diseases, it is found that in the 288 tomato samples, 247 and 235 tomato samples have specific primer binding site respectively. Then, restriction enzymes (TaqI and HincII) were used to understand whether the individuals were homozygote or heterozygote resistant, or susceptible to these diseases for determination of resistance to root-knot nematodes and Verticillium wilt. It is found that 200 samples were susceptible, 45 samples were heterozygote resistant and 2 samples were homozygote resistant to root-knot nematodes, and 125 samples were susceptible, 110 samples were heterozygote to Verticillium wilt