Esin POYRAZOĞLU ÇOBAN, H. Halil BIYIK, Bahadır TÖRÜN

Investigation of Antimicrobial Effects in Different Solvents of Pistacia terebenthus L. Fruits Collected from Aydın

In this study, the antimicrobial activity of Pistacia terebenthus L. fruits extracts were tested against some pathogen microorganisms. Fruits of the plant samples were freeze-dried and powdered. 10 grams of this material was extracted separately in 150mL of methanol, ethyl acetate, and boiled water for 6 h. at Soxhlet. The extracts were concentrated and then kept at 4°C. The agar well diffusion method is used for testing the antimicrobial activities of extracts. The results of methanol extract of Pistacia terebenthus L. fruits was found to form larger zones against tested bacterial pathogens E. coli ATCC 35218,S. aureus ATCC 25923, S. typhimiriumATCC 14028, M. smegmatis ATCC 607, C. xerosis ATCC 373. In addition the ethyl acetate and boiled distilled water extracts of Pistacia terebenthus L. fruits demonstrated moderate effect against some bacterial pathogens tested. However, none of the extracts used showed any effect on yeast and molds.The ethyl acetate extract of Pistacia terebenthus L. fruits have effects on wider spectrum than methanol and boiled water extracts for revealing secondary metabolites like alkaloids, tannins, and flavonoids.

Keywords:

Pistacia terebenthusfruits extracts, antimicrobial effect, agar well diffusion method,

PDF

___

[1]Törün B, Çoban EP, Biyik HH, Barisik E. 2017. Antimicrobial Activity of Echinophora tenuifolia L. and Raphanus sativus L. Extracts. Indian J Pharm. Education Research. 51(1):136-43.
[2] Singh N, Gupta S, Rathore V. 2017. Comparative antimicrobial study of ethanolic extract of leaf and rhizome of Curcuma longa Linn. Pharmacognosy J. 9(2):208-12.
[3]Badakhshan MP, Sasidharan S, Rameshwar NJ, Ramanathan S. 2009. A Comparative study: Antimicrobial activity of methanol extracts of Lantana camara various parts. Phcog. Res. 1(6):348-51.
[4] Tepe B, Daferera D, Sökmen M, Polissiou M, Sökmen A. 2004. In vitro antimicrobial and antioxidant activities of the essential oils and various extracts of Thymus eigii. J Agric Food Chem. 52(1):132-7.
[5] Bankole MA, Shittu LA, Ahmed TA, Bankole MN, Shittu RK, Kpela T. 2007. Synergistic antimicrobial activities of phytoestrogens in crude extracts of two sesame species against some common pathogenic microorganisms. Afr J Trad Comp Alternative Med. 4(4):427-33.
[6] Nag M, Mukherjee P, Biswas R, Chanda J, Kar A. 2016. Evaluation of antimicrobial potential of some Indian ayurvedic medicinal plants. Pharmacognosy J. 8(6):525-33.
[7] Roumy V, Gutierrez-Choquevilca AL, Lopez Mesia JP, Ruiz L, Ruiz Macedo JC, Abedini A, et al. 2015. In vitro antimicrobial activity of traditional plant used in mestizo shamanism from the Peruvian Amazon in case of infectious diseases. Phcog Mag. 11(4): 625-33.
[8]Çoban EP, Bıyık HH, Uzun C. 2009. Investigation of antimicrobial activity of some natural plants which are not cultivated and are sold at bazaars in Aydın. Int J Nature Eng Sci. 3:59-62.
[9]Collins CH, Lyne PM, Grange JM, Falkinham JO. 2004. Microbiological methods. 8th ed. London: Arnold Publishers.
[10]Clinical and Laboratory Standards Institute. CLSI. 2015. M02-A12: performance standards for antimicrobial disk susceptibility tests: approved standard. 12.ed. Wayne: CLSI, p.1-73.
[11]Kavak DD, Altıok E, Bayraktar O, Ülkü S. (2010). Pistacia terebinthus extract: As a potential antioxidant, antimicrobial and possible -glucuronidase inhibitor. J Molecular Catalysis B: Enzymatic. 64: 167–171.
[12] Durak MZ, Uçak G. (2015). Solvent optimization and characterization of fatty acid profile and antimicrobial and antioxidant activities of Turkish Pistacia terebinthus L. extracts. Turk J Agric For. 39: 10-19.
[13]Bledar P, Behxhet M, Kate N, Cassandra LQ, Avni H. 2016. Chemical composition and in vitro antibacterial activity of Pistacia terebinthus essential oils derived from wild populations in Kosovo. BMC Complementary Alternative Med. 16(147): 1-9.
[14]Coban EP, Bıyık H, Törün B, Yaman F. (2017). Evaluation the antimicrobial effects of Pistacia terebinthus L. and Papaver rhoeas L. extracts against some pathogen microorganisms. Indian J Pharm Education Research. 51(3): 377-380.
[15] Haliki A, Çetingül V, Denizci AA. 1998. An Investigation on Antibacterial Activities of Some Marine Algae (Phaeophyta, Rhodophyta), Ege UniversityJournal Of The Faculty Of Science, 21; 1-9.