In vitro Regeneration of Vanilla (Vanilla planifolia L.)

Vanilla is a high demanding product sold at high prices in world markets. Natural vanilla has high economic value and is theworld’s second most expensive spices after saffron. Despite its economic value, it has been one of the least studied species of vanilla plants among other orchid species. Vanilla’s synthetic form (vanillin) can be obtained from various chemical methods by using starting materials (coniferin, guaiacol, eugenol and lignin), but the vanilla flavor can naturally be obtained only from vanilla fruit. Micropropagation is an alternative technique for economically important plant production more effectively used with minimum starting material. For this reason, in the study, regeneration of the vanilla plant in vitro were carried out. Seed, young leaves, node and maerial parts of V. planifolia L. were used as an initial material. The highest callus regeneration (80%) from node explants was obtained in VK4 (MS+1 mg/L 2,4-D + 1 mg/L BAP) nutrient medium, while 100% direct shoot regeneration was provided in VK3 (MS+1 mg/L 2,4-D + 0,5 mg/L BAP) and VK6 (MS+1 mg/L BAP + 0,5 mg/L NAA) nutrient media. In addition, 50% rooting percentage was obtained in VK6 medium. Indirect shoot regeneration ratio was determined as 33,3% in VK2 (MS + 1 mg / L 2,4-D + 0,5 mg / L KIN) and 71,4% of indirect embryo formation was detected in VK4 medium. All embryos stayed in globules stages

Keywords:

vanilla Vanilla planifolia L., regeneration, in vitro,

PDF

___

[1] G. Ravishankar, R. R. a. “Vanilla flavour: production by conventional and biotechnological routes,” J Sci Food Agric, vol. 80, pp. 289-304, 2000.
[2] Sharrine Omari Domingues de Oliveira, R. M. S. T. A. B. J. E. S.-P. “A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system,” Scientia Horticulturae, no. 161, p. 204–209, 2013.
[3] Krushnamurthy Anuradha, B. N. S. &. M. M. N. “Vanilla- Its Science of Cultivation, Curing, Chemistry, and Nutraceutical Properties, Critical Reviews in Food Science and Nutrition.,” vol. 53, p. 250–1276, 2013.
[4] Isidore Mushimiyimana, T. A. C. D. F. G. J. N. V. A. J. K. a. D. G. “In Vitro Propagation of Vanilla in Rwanda,” Rwanda Journal, vol. 24, 2011.
[5] A. Anilkumar, “ Vanilla cultivation. A profitable agri-based enterprise,” KERALA CALLING, College of Agriculture, Vellayani, 2004.
[6] Zerihun Abebe, A. M. A. T. a. W. T. “Efficient in vitro multiplication protocol for Vanilla planifolia using nodal explants in Ethiopia,” African Journal of Biotechnology, vol. 8, no. 24, pp. 6817-6821, 2009.
[7] L. Møller,N. J. G. a. B. “Vanillin–Bioconversion and Bioengineering of the Most Popular Plant Flavor and Its De Novo Biosynthesis in the Vanilla Orchid,” Molecular Plant, vol. 8, p. 40–57, 2015.
[8] Minoo Divakaran, K. N. B. P. R. a. K. P. “Biotechnology for micropropagation and enhancing variations in Vanilla,” Asian J. Plant Sci. Res., vol. 5, no. 2, pp. 52-62, 2015.
[9] Jean Gabriel Fouché, L. J. “Vanilla planifolia: history, botany and culture in Reunion island,” Agronomie, vol. 19, pp. 689-703, 1999.
[10] E. D. Bello, “Vanillin production from ferulic acid with Pseudomonas fluorescens BF13-1p4,” 2013.
[11] S. R.V., “Novel approaches for Molecular Analysis, Micropropagation and Curring of Vanilla (Vanilla planifolia),” 2009.
[12] Borges da Silvaa E.A., Z. M. A. J. C. C. B. M. B. M. R. A. “An Integrated Process to Produce Vanillin and Lignin-based Polyurethanes from Kraft Lignin,,” chemical engineering research and design, vol. 8, no. 7, p. 1276–1292, 2009.
[13] Yanjun Zhang, L. M. F. C. M. L. W. D. Q. W. F. X. a. F. G. “Zhang Y., Mo L., Chen F.,Optimized Production of Vanillin from Green Vanilla Pods by Enzyme-Assisted Extraction Combined with Pre-Freezing and Thawing,” Molecules, vol. 19, pp. 2181-2198, 2014.
[14] J. T. Wong, “Technological, Commercial, Organizational, And Social Uncertainties Of A Novel Process For Vanillin Productıon From Ligni,” 2012.
[15] L. Dressler, M. A. S. A. &. R. “A Revision of The Mexican And Central American Species of Vanilla Plumier Exmiller with A Characterızatıon Of Their Its Region Of The Nuclear RibosomaL DNA,” Lankesteriana, vol. 9, no. 3, p. 285—354., 2010.
[16] Tony L Palama, P. M. I. F. Y. H. C. E. B. J. G.-S. M. B. B. P. R. V. a. H. K. “RSehseaorcoh atr tdicleifferentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage,” BMC Plant Biology, vol. 10, no. 82, pp. 1471-2229, 2010.
[17] S. Seshadri & B. J. “Plantlet regeneration from leaf derived callus of Vanilla planifolia Andr,” in vitro Cell. Dev.Biol.-Plant, vol. 44, p. 84–89, 2008.
[18] Boon Chin Tan, C. F. C. P. A. “Optimisation of plantlet regeneration from leaf and nodal derived callus of Vanilla planifolia Andrews,” Plant Cell Tiss Organ Cult, vol. 105, p. 457–463, 2011.
[19] S. Bora, R. S. “Comparative Studies on Callus Induction from Different Explants of Vanilla planifolia Andrews,” International Journal of Advanced Biotechnology and Research, vol. 6, no. 3, pp. 360-365, 2015.
[20] Emine BAYRAM, S. K. S. T. G. Y. O. A. S. K. İ. T., “Tıbbi Ve Aromatik Bitkiler Üretiminin Arttırılması Olanakları,” 2009.
[21] Vujanovic V., S.-A. M. B. D. a. T. G. “Viability Testing of Orchid Seed and the Promotion of Colouration and Germination,” Annals of Botany, vol. 86, pp. 79-86, 2000.