This study reports a protocol about embryogenic callus propagation in Triticum monococcum ssp. monococcum using zygotic embryos. Embryogenic callus induction was noted on all concentrations of 2,4-D used in this study. Maximum embryogenical callus induction in terms of embryogenic callus weight was noted on MS medium containing 4 mg/l 2,4-D. Minimum embriyogenic callus weight was noted on MS medium containing 8 mg/l 2,4-D. Increase or decrease of 2,4-D concentratin resulted in decrease in embryogenic callus weight