This study has been conducted to investigate different explants of plant growth regulators on callus induction of Calendula officinalis L. medicinal and aromatic herb. The seeds were surface sterilized using 70% ethanol for 3 minutes, 10% commercial bleach for 5 minutes and rinsed 3 times with sterile water for 3 minutes. The seeds were germinated in Murashige and Skoog (MS) medium. Hypocotyl, cotyledon and cotyledon node explants have been excised from the plantlets obtained from in vitro germinated seeds. Three explants were cultured on MS media supplemented with various concentrations of cytokinin (BAP; 1.0, 2.0 mgL-1) and auxin (IBA; 0.1, 0,5 mgL-1) for callus induction. At the end of eight weeks, the best results were observed in a treatment with 1 mgL100% on the hypocotyl; 1 mgL-1 BAP + 0.5 mgL-1 IBA and 2 mgL-1 BAP + 0.5 mgL-1 IBA 88% on the cotyledon; 2 mgL-1 BAP + 0.1 mgL-1IBA 100% on the cotyledon nodes. The data obtained from the path is instructive to gene transformation, phytoremediation and cell culture study