The present work aims to demonstrate the profound effect of a widely used fungicide Thiovit® Jet, on several life history traits in Drosophila melanogaster. The fungicide under study has a very wide application in fruit orchards. Freshly laid eggs of wild type D. melanogaster, were transferred to different dietary concentrations of the test chemical (20, 30, 40, 50, 100, 200, 400, 800, 1600 and 2000ppm). Larval duration, Pupal duration and Emergence of flies of both treated and control groups were recorded. Results obtained show a significant (p<0.05) change in the mentioned parameters in the treated flies in comparison to the control. The maximum noticeable increase in duration has been recorded with 40ppm treatment. Thus the study indicates an effect of a fungicide Thiovit® Jet on the duration of different life stages and thereby suggesting a role of the fungicide on a non-target organism like Drosophila