Model Organizma Galleria mellonella L.’da Bakır Nanopartiküllerinin Oksidatif Stres Üzerine Etkilerinin Değerlendirilmesi

Yapılan çalışmada farklı derişimlerdeki bakır oksit nanopartiküllerinin (10, 100 ve 1000 µg/mL) Galleria mellonella larvalarının orta barsak ve yağ dokusundaki katalaz (CAT), superoksit dismutaz (SOD), glutatyon peroksidaz (GPx), glutatyon-s-transferaz (GST) ve asetilkolinesteraz (AChE) aktiviteleri üzerine etkileri araştırılmıştır. Farklı derişimlerdeki bakır oksit nanopartiküllerine maruz bırakılan G. mellonella larvalarının orta barsak ve yağ dokularında CAT, SOD ve AChE aktivitelerinde azalma tespit edilirken, 100 µg/mL bakır oksit nanopartikülü uygulaması yapılan grupta GPx aktivitelerinde artış meydana geldiği belirlenmiştir. Ayrıca GST aktivitesinde tüm uygulama gruplarında yağ dokuda azalma, orta barsakta ise artış meydana geldiği tespit edilmiştir. Genel olarak, bu bulgular, G. mellonella larvalarının bakır oksit nanopartikülünün toksisitesi sonucunda reaktif oksijen türlerinin birikimi nedeniyle oksidatif stresin meydana geldiğini göstermektedir.

Anahtar Kelimeler:

Antioksidan enzimler, bakır nanopartikülleri, Galleria mellonella

Evaluation of the effects of copper nanoparticles on oxidative stress in the model organism Galleria mellonella L.

In this study, the effects of copper oxide nanoparticles at different concentrations (10, 100 and 1000 µg/mL) on catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-s-transferase (GST) and acetylcholinesterase (AChE) activities were investigated in the midgut and fat body of Galleria mellonella larvae. It was determined that GPx activities increased in the group exposed to 100 µg/mL copper oxide nanoparticles, whereas a decrease in CAT, SOD and AChE activities was observed in midgut and fat body of G. mellonella larvae exposed to all copper oxide nanoparticles applied groups. Moreover, GST activity decreased in fat body in all applied groups, while an increased was observed in midgut of G. mellonella larvae when exposed to copper oxide nanoparticles. Overall, these findings indicate that oxidative stress occurs due to the accumulation of reactive oxygen species as a result of the toxicity of the copper oxide nanoparticle of G. mellonella larvae.

Keywords:

Antioxidant enzymes, copper nanoparticles, Galleria mellonella,

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