Osman SERDAR, Nuran CIKCIKOĞLU YILDIRIM, Şule TATAR, Numan YILDIRIM

Kurşuna Maruz Bırakılan Gammarus pulex’de Antioksidan Enzim Yanıtları

Bu çalışmada, kurşuna maruz bırakılan G. pulex'de süperoksit dismutaz, katalaz ve glutatyon peroksidaz antioksidan enzimlerinin aktivite yanıtlarının araştırılması amaçlanmaktadır. G. pulex, 96 saat boyunca 10, 30, 50 µg/L kurşun içeren sentetik çözeltilere maruz bırakılmıştır. Süperoksit dismutaz, katalaz ve glutatyon peroksidaz aktiviteleri ELISA kiti kullanılarak belirlenmiştir. Süperoksit dismutaz aktivitelerindeki değişimler istatistiksel olarak anlamlı bulunmamıştır (P>0.05). Kurşuna maruz bırakıldıktan sonra tüm uygulama gruplarında katalaz aktiviteleri azalmıştır (P<0.05). Glutatyon peroksidaz aktiviteleri kurşun maruziyetine bağlı olarak azalmıştır (P>0.05). Bulgularımız, reaktif oksijen türleri üreterek ve sülfhidril bağımlı antioksidan enzim aktivitelerini inhibe ederek kurşunun oksidatif strese neden olabileceğini göstermektedir. Sonuç olarak, antioksidan enzimlerdeki değişiklikler, kurşunun çevresel risk değerlendirmesi için potansiyel olarak hassas biyobelirteçler olarak kullanılabilir ve deşarj standartlarının oluşturulmasına katkıda bulunabilir.

Anahtar Kelimeler:

G. pulex, Süperoksit Dismutaz, Katalaz, Glutatyon Peroksidaz

Antioxidant Enzyme Responses to Lead Exposure of Gammarus pulex

It is aimed to investigate the activity response of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase of G. pulex exposed to lead. The G. pulex were exposed synthetic solutions containing 10, 30, 50 µg/L lead for 96 h. Superoxide dismutase, catalase, glutathione peroxidase activities were determined by using ELISA kit. The differences in superoxide dismutase activities were statistically insignificant (P>0.05). Catalase decreased in all treatment groups after lead exposure (P<0.05). The glutathione peroxidase activities were decreased depending on lead exposure (P>0.05). Our findings suggest that lead can induce oxidative stress by generating reactive oxygen species and inhibiting sulfhydryl dependent antioxidant enzymes activities. In conclusion, alterations in antioxidant enzymes may potentially be used as sensitive biomarkers for risk assessment of lead in the environment and may contribute to the establishment of discharge regulations.

Keywords:

G. pulex, Superoxide Dismutase, Catalase, Glutathione Peroxidase,

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