Gebe Sığırlara İnaktif Escherichia coli Aşısı Uygulamasının Kolostrum ve Buzağı Kan Serumlarındaki IgG Seviyelerine Etkisinin Araştırılması

Bu çalışmada, gebe sığırlara uygulanan inaktif E. coli aşısının kolostrum ve buzağı kan serumlarındaki immunoglobulin (Ig) G seviyelerine olan etkisinin araştırılması amaçlandı. Bu amaçla, gebeliklerinin farklı dönemlerindeki sığırlara (son 60 ve 30 gün) ticari bir inaktif E. coli aşısı uygulandı. Aşılanan sığırların kolostrumu ve bu kolostrumlarla beslenen buzağıların kan serumlarındaki IgG seviyeleri ELISA ile incelendi. Aşılama grupları ve kontrol grubu arasında IgG seviyelerindeki farklılıklar ölçülerek, gruplar arasındaki IgG değerleri karşılaştırıldı. Kolostrum örneklerine ait veriler incelendiğinde, kontrol grubu ile tek doz ve iki doz aşılanan hayvanlardan elde edilen sonuçlar arasındaki farkın önemli (p<0,001) olduğu belirlendi. Benzer şekilde buzağı serumlarına ait veriler değerlendirildiğinde; tek doz ve iki doz aşı yapılanan sığırların buzağılarında ölçülen kan serumu değerlerinde kontrol grubuna göre önemli oranda artış tespit edildi (p<0,001). Diğer yandan kontrol grubundaki 7 buzağının tamamında (%100), tek doz aşılanan sığırlara ait 7 buzağının 5’inde (%71,4) pasif transfer yetersizliği (PTY) görülürken, iki doz aşılanan 7 buzağının hiçbirinde PTY belirlenemedi. Böylece aşılanan hayvanlarda PTY oranlarında önemli oranda düşüş sağlanmış oldu. Sonuç olarak gebe sığırlara uygulanan inaktif E. coli aşısı ile oluşturulan immun yanıtın pasif olarak buzağılara transfer edildiği, gebe sığırların gebeliklerinin son 60 ve 30. gününde iki doz aşılamanın, tek doz aşılamaya göre daha etkili olduğu kanaatine varıldı.

Anahtar Kelimeler:

Blood sera, Bovine, Colostrums, ELISA, Inactive E. coli vaccine, IgG

Investigation of Effect of Inactive Escherichia coli Vaccination on Pregnant Cattle IgG Levels in Colostrums and Calf Blood Serums

In this study, it was aimed to investigate the effect of inactive E. coli vaccine administered to pregnant cattle on immunoglobulin (Ig) G levels in colostrums and calf blood serum. For this purpose, a commercial inactive E. coli vaccine was administered to cattle at different stages of their pregnancy (last 60 and 30 days). The colostrums of vaccinated cattle and IgG levels in blood serum of calves fed with these colostrums were analyzed by ELISA. Differences in IgG levels between vaccination groups and control groups were measured and IgG values between groups were compared. When the data of the colostrum samples were examined, it was determined that the difference between the results obtained from the control group and the animals that were vaccinated with one dose and two doses was significant (p <0.001). Similarly, when the data of calf sera are evaluated; There was a significant increase in blood serum values measured in calves of cattle receiving single dose and two doses of vaccine compared to the control group (p <0.001). On the other hand, passive transfer failure (PTF) was observed in all 7 calves (100%) in the control group and in 5 (71.4%) of the 7 calves that were vaccinated with a single dose, while PTF could not be determined in any of the 7 calves that were vaccinated with two doses. Thus, a significant decrease in PTF rates was achieved in vaccinated animals. As a result, it was concluded that the immune response generated by the inactive E. coli vaccine administered to pregnant cattle was transferred to calves passively, and that two doses of vaccination on the last 60 and 30 days of pregnancy of pregnant cattle were more effective than single dose vaccination.

Keywords:

ELISA, İnaktif E. coli aşısı, IgG, Kan serumu, Kolostrum, Sığır,

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