Bacillus subtilis ve Lactobacillus plantarum Probiyotik Bakterilerinin Bazı Balık Patojenleri Üzerine in vitro Antagonistik Etkisi

In vitro Antagonistic activity of Probiotic Bacteria Bacillus subtilis and Lactobacillus plantarum on Fish Pathogens

Bu çalışmada iki potansiyel probiyotik bakteri Bacillus subtilis ve Lactobacillus plantarum’ un bazı balık patojenlerine karşı antagonistik etkisi araştırılarak, su ürünleri yetiştiriciliğinde kullanılabilirlikleri değerlendirilmiştir. Bu amaçla Aeromonas sobria, Citrobacter sp., Lactococcus garvieae, Listonella anguillarum, Edwardsiella tarda, ve Yersinia ruckeri patojenleri üzerine probiyotiklerin ürettikleri antibakteriyel maddelerin etkisi sıvı kültür inhibisyon testi ile in vitro olarak değerlendirilerek antagonistik etkinin varlığı araştırılmıştır. Sıvı kültür inhibisyon testi iki probiyotik türünün üst fazının 36 saatlik kültür sonucunda bazı balık patojenlerinin çoğalmasını inhibe edebildiğini göstermiştir. Test sonuçları B. subtilis'in A. sobria, E. tarda ve L. garviae'nin inhibisyonunda etkili olduğunu göstermiştir. L. plantarum probiyotiğinin A. sobria, L. garviae, L. anguillarum ve Y. ruckeri'nin büyümesini engellediği tespit edilmiştir. İleriki çalışmalarda Bacillus subtilis ve Lactobacillus plantarum probiyotik bakterilerinin balık yemlerine ilave edilerek besleme çalışmalarının yapılmasına ve bu çalışmada test edilen patojenlere karşı balıkların direnç kazanımlarının araştırılmasına ihtiyaç vardır.

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