Akut Toksisite Testi Hassasiyetinin Belirlenmesinde Referans Toksik Madde Olarak Formaldehit Kullanımı

Hızlı toksisite testlerinden; test süresi, numune hacmi, uygun maliyeti ve sonuç hassasiyeti nedeniyle; biyolüminesans bakteri ile yapılan akut toksisite testi, birçok ülke standartlarında yerini almıştır. Biyolüminesans bakterinin ticari bir kiti olan Microtox® Reagent’ın hassasiyeti, referans toksik madde (kontrol) sayesinde tespit edilebilmektedir. Microtox® akut toksisite testinde kullanılmak üzere üretici firma tarafından farklı referans toksik maddeler (kontrol) önerilmektedir. Bu çalışmada, Microtox® akut toksisite testinde kullanılmak üzere formaldehitin alternatif referans toksik madde (kontrol) olarak kullanımı hedeflenmiştir. Formaldehitin Vibrio fischeri’ye göre olan akut toksisitesi, 5, 15 ve 30. dakika inhibisyon sürelerinde çalışılmıştır. İnhibisyon sürelerine göre EC50 değerleri sırasıyla 2,30±0,60 mg/l; 2,14±0,63 mg/l ve 2,17±0,70 mg/l bulunmuştur. Her bir örnekleme grubu için bulunan EC50 değerlerinin, ortalamadan sapmaları ise 0,01-0.47 arasında değişim göstermiştir. Formaldehitin tüm inhibisyon süreleri için ortalama akut toksisite sonucuna bakıldığında da; 5. dakikalık sonuçta 0,10 birim sapma görülmektedir. 15 ve 30 dakikalık inhibisyon süreleri için ise; ortalama sırasıyla 0,06 ve 0,04 birim sapma ile ortalamaya ve birbirlerine daha yakın değerler aldığı tespit edilmiştir. Çalışılan referans toksik maddeler; reagent, analizör ve test operatörünün pipetleme hassasiyetinin performansını göstermektedir.

Anahtar Kelimeler:

Akut toksisite, Vibrio fischeri, Microtox®, referans toksik madde, formaldehit

Interlaboratory Precision of Acute Toxicity Tests Using Reference Toxicant Formaldehyde

Acute toxicity test, which takes place among rapid toxicity tests and is conducted through bioluminescent bacteria, has been included in many countries' standards thanks to its test duration, sample volume, cost-efficiency and result sensitivity. Sensitivity of Microtox® Reagent, being one of the commercial kits of bioluminescent bacteria, can be detected via reference toxicants (control). Various reference toxicants (control) are recommended by the manufacturer company to be used in Microtox® acute toxicity test. This study aims to use formaldehyde as alternative reference toxicant (control) in Microtox® acute toxicity test. Acute toxicity of formaldehyde, according to Vibrio fischeri, was investigated on 5, 15 and 30 minutes of inhibition periods. EC50 values were found as 2,30±0,60 mg/l; 2,14±0,63 mg/l and 2,17±0,70 mg/l by inhibition periods, respectively. Standard deviation of EC50 values found for each sample group differed between 0,01-0,47. According to mean acute toxicity results of all formaldehyde inhibition periods, 0,10 unit deviation was found in 5 minutes of results. For 15 and 30 minutes of inhibition periods, mean value of unit deviation was found as 0,06 and 0,04 which showed closer values to each other and the mean value. The investigated toxicants show the performance of pipetting sensitivity of reagent, analyzer and test operator.

Keywords:

Acute toxicity, Vibrio fischeri, Microtox®, reference toxicant, formaldehyde,

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