MS-222, propofol ve karanfil yağının Daphnia magna’ larda anestezik etkisinin kalp atım oranlarına göre değerlendirilmesi

Günümüzde Avrupa Birliği (EU), Amerikan Test ve Malzeme Kurumu (ASTM), Uluslararası Standardizasyon Teşkilatı (ISO), Ekonomik Kalkınma ve İşbirliği Örgütü (OECD) gibi pek çok kuruluş; bazı kimyasal maddelerin, ilaçların gerek çevreye, gerekse canlılara olan etkilerini invivo koşullarda araştırılması amacıyla Daphnia magna adı verilen sucul omurgasız canlıdan yararlanılmaktadır. Su ürünleri yetiştiriciliğinde karanfil yağı, MS 222 ve propofol yaygın bir şekilde kullanılan anestezik maddelerdendir. Ancak bu anestezik maddelerin tatlı su ortamında bulunan kabuklu canlılar için etkileri hakkında çok fazla bilgi yoktur. Yapılan bu çalışmada, anestezik etkisi olan, MS-222, propofol, ve karanfil yağının su piresi olarak bilinen Daphnia magna’ larda kısa süredeki kalp atım sayısında oluşturduğu farklar ve hayatta kalma etkisi araştırılmıştır. Anestezik maddeler 0.1-0.01-0.001 ml/L olarak uygulanmış ve çalışmanın 1., 3., ve 5. saatlerinde su pirelerinin kalp atım sayıları ve ölüm oranları değerlendirilmiştir. Deneme süresince elde edilen verilere göre kullanılan üç farklı anestezik maddenin kalp atım sayıları ve ölüm oranları üzerindeki etkileri bakımından anlamlı bir fark olduğu görülmüştür (p<0.05). Çalışmanın sonucu olarak düşük oranlarda bile bu üç farklı anestezik maddenin tatlı su ortamlarında bulunan omurgasız canlılar için toksik bir etki yaratabileceği ve ekolojik dengeyi etkileyebileceği görülmüştür.

Anahtar Kelimeler:

Anesteziyoloji, karanfil yağı, MS 222, propofol, su piresi, toksikoloji

Evaluation of the anesthetic effect of MS-222, propofol and clove oil in Daphnia magna according to heart rate

Today, many organizations such as the European Union (EU), American Testing and Materials Agency (ASTM), International Organization for Standardization (ISO), Economic Development and Cooperation Organization (OECD); An aquatic invertebrate called Daphnia magna is used to investigate the effects of certain chemicals and drugs on both the environment and living things in vivo. Clove oil, MS 222 and propofol are commonly used anesthetics in aquaculture. However, there is not much information about the effects of these anesthetic agents for crustaceans in the freshwater environment. In this study, the effects of MS-222, propofol, and clove oil, which have anesthetic effects, in the short-term heart rate and survival effect of Daphnia magna, known as the water flea, were investigated. Anesthetic agents were administered as 0.1-0.01-0.001 ml/L, and heart rate and death rates of water fleas were evaluated at the 1st, 3rd, and 5th hours of the study. According to the data obtained during the trial, there was a significant difference in the effects of three different anesthetic agents on heart rate and death rates (p<0.05). As a result of the study, it has been seen that these three different anesthetic substances can create a toxic effect for invertebrates in freshwater environments and affect the ecological balance, even at low rates.

Keywords:

Anesthesiology, clove oil, daphnia, MS 222, propofol, toxicology,

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