Funda TERZİ, Ayşe Gül DAL, Serdar ODACI, Yavuz ULUSOY, Bahadır KILINÇ

Investigation of respiratory syncytial virus (RSV) and parainfluenza-3 (PI-3) virus by histopathological and immunohistochemical methods in sheep and goat

Koyun ve keçilerde Respiratuar Sinsitiyal Virüsü (RSV) ve Parainfluenza tip 3 (PI3) virüsü ciddi solunum sistemi enfeksiyonlarına neden olmaktadır. Çalışmada, koyun ve keçi RSV ve PI-3 virüsünün akciğer dokusundaki yerleşimi ve dağılımının histopatolojik ve immünohistokimyasal yöntemlerle araştırılması amaçlandı. Çalışma materyalini, 2016-2019 yılları arasında, Samsun ve çevre yörelerinden Samsun Kontrol ve Veteriner Araştırma Enstitüsüne gelen 30 adet koyun ve 24 adet keçi akciğer parafin blok dokusu oluşturdu. Akciğer parafin blok dokularının histopatolojik incelemesinde, bronş ve bronşiyol epitelinde dejenerasyon ve dökülme, interalveolar septumda kalınlaşma, epitelizasyon ve alveolar makrofajlarda artış yaygın olarak görülürken, lenfoid doku da hiperplazi, hiyalin membran oluşumu ve sinsityal hücrelere daha az oranda rastlandı. Ayrıca hiçbir olguda bronşiolitis obliteransa rastlanmadı. İmmünhistokimyasal olarak, koyunların %50'sinde ve keçilerin %54'ünde RSV antijeni, koyunların %40'ında ve keçilerin %50'sinde PI-3 antijeni tespit edildi. Koyun ve keçi akciğer parafin kesitlerinde RSV ve PI-3 antijeni, bronş ve bronşiol epitel hücreleri ile lümende dökülmüş hücrelerinde ve interalveolar septum hücrelerinde istatistiksel olarak benzerdi (P>0.05). Alveolar makrofajlarda keçilerde PI-3 antijeni, koyunlara göre istatistiksel olarak daha yoğun boyandı(P<0.05). Koyun ve keçi akciğer dokusunda RSV ve PI-3 antijeninin replikasyon alanları benzer olduğu kanısına varıldı. Koyun ve keçi akciğer dokusunda, RSV ve PI-3 virusun neden olduğu sitokin yanıt mekanizmasının moleküler yöntemlerle araştırılması gerektiği düşünülmektedir.

Keywords:

RSV, PI-3, Immunohistochemistry, Sheep, Goat.,

PDF

___

Al-Hammadi, M. A. (2016): Serological Surveillance of Infectious Bovine Rhinotracheitis virus, Bovine viral diarrhea virus and Bovine Parainfluenza-3 virus in Saudi Arabia. Alexandria Journal for Veterinary Sciences, 51(1), 48-53. https://doi.org/https://doi.org/10.5455/ajvs.238696
Aniță, A., Aniță, D., Răileanu, C., & Savuța, G. (2015): Detection of Parainfluenza Type 3 Virus Antigens in Goats. Bulletin UASVM Veterinary Medicine, 72(1), 27-29. https://doi.org/http://dx.doi.org/10.15835/buasvmcn-vm:10385
Belknap, E. B., Ciszewski, D. K., & Baker, J. C. (1995): Experimental respiratory syncytial virus infection in calves and lambs. J Vet Diagn Invest, 7(2), 285-298. https://doi.org/https://doi.org/10.1177/104063879500700226
Borujeni, M. P., Hajikolaei, M. R. H., Shapouri, M. R. S. A., & Roshani, F. (2020): The role of sheep in the epidemiology of Bovine alphaherpesvirus 1 (BoHV-1). Preventive veterinary medicine, 174, 104818. https://doi.org/https://doi.org/10.1016/j.prevetmed.2019.104818
Bryson, D., McNulty, M., McCracken, R., & Cush, P. (1983): Ultrastructural features of experimental parainfluenza type 3 virus pneumonia in calves. J COMP PATH, 93(3), 397-414. https://doi.org/https://doi.org/10.1016/0021-9975(83)90027-0
Bryson, D., Platten, M., McConnell, S., & McNulty, M. (1991): Ultrastructural features of lesions in bronchiolar epithelium in induced respiratory syncytial virus pneumonia of calves. Vet Pathol, 28(4), 293-299. https://doi.org/https://doi.org/10.1177/030098589102800405
Caswell, J. L., & Williams, K. (2007). The respiratory system. In M. G. Maxie (Ed.), Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals (Vol. 5, pp. 594–622). Saunders Elsevier.
Ceribasi, A., Ozkaraca, M., Ceribasi, S., & Ozer, H. (2014): Histopathologic, immunoperoxidase and immunofluorescent examinations on natural cattle pneumonia originated from Parainfluenza type 3, Respiratory Syncytial virus, Adenovirus type 3 and Herpesvirus type 1. Revue Med Vet, 165, 201-212.
Ceribasi, S., Ozkaraca, M., Ozer, H., & Ceribasi, A. (2013): Diagnosing respiratory syncytial virus using immunofluorescence and immunohistochemistry methods in caprine lungs with bronchopneumonia. Rev Méd Vét, 164, 120-124.
Ceribasi, S., Özkaraca, M., Özer, H., & Çeribasi, A. O. (2012): Prevalence of parainfluenza type 3 virus antigens in pneumonic caprine lungs by direct immunofluorescence and immunoperoxidase techniques. Small ruminant research, 108(1-3), 127-132. https://doi.org/https://doi.org/10.1016/j.smallrumres.2012.07.033
Eleraky, N. Z., Kania, S. A., & Potgieter, L. N. (2001): The ovine respiratory syncytial virus F gene sequence and its diagnostic application. J Vet Diagn Invest, 13(6), 455-461. https://doi.org/https://doi.org/10.1177/104063870101300601
Ellis, J. A. (2010): Bovine parainfluenza-3 virus. Vet Clin Food Anim, 26(3), 575-593. https://doi.org/https://doi.org/10.1016/j.cvfa.2010.08.002
Emikpe, B. O., Jarikre, T. A., Akpavie, S. O., Opoku-Agyemang, T., Asare, D., & Folitse, R. D. (2019): Histological and immunohistochemical assessments of pneumonia in sheep slaughtered at Ibadan, Nigeria and Kumasi, Ghana. Journal of Immunoassay and Immunochemistry, 40(3), 300-313. https://doi.org/ https://doi.org/10.1080/15321819.2019.1589495
Franco, M. F., Gaeta, N. C., Alemán, M. A. R., Nogueira, A. H. C., Pituco, E. M., Balaro, M. F. A., & Gregory, L. (2020): Indirect detection of respiratory viruses responsible for respiratory disease in sheep. Medicina Veterinária (UFRPE), 14(1), 7-13. https://doi.org/https://doi.org/10.26605/medvet-v14n1-2582
Haanes, E. J., Guimond, P., & Wardley, R. (1997): The bovine parainfluenza virus type-3 (BPIV-3) hemagglutinin/neuraminidase glycoprotein expressed in baculovirus protects calves against experimental BPIV-3 challenge. Vaccine, 15(6-7), 730-738. https://doi.org/https://doi.org/10.1016/S0264-410X(96)00231-9
Haines, D. M., Kendall, J. C., Remenda, B. W., Breker-Klassen, M. M., & Clark, E. G. (1992): Monoclonal and polyclonal antibodies for immunohistochemical detection of bovine parainfluenza type 3 virus in frozen and formalin-fixed paraffin-embedded tissues. J Vet Diagn Invest, 4(4), 393-399. https://doi.org/https://doi.org/10.1177/104063879200400404
Jarikre, T. A., & Emikpe, B. O. (2017): First report of immunohistochemical detection of peste des petit ruminants, parainfluenza 3 and respiratory syncytial viral antigens in lungs of Nigerian goats. Journal of Immunoassay and Immunochemistry, 38(5), 555-568. https://doi.org/https://doi.org/10.1080/15321819.2017.1349669
Maidana, S. S., Lomonaco, P. M., Combessies, G., Craig, M. I., Diodati, J., Rodriguez, D., Parreño, V., Zabal, O., Konrad, J. L., & Crudelli, G. (2012): Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina. BMC veterinary research, 8(1), 83. https://doi.org/http://www.biomedcentral.com/1746-6148/8/83
Masot, A., Gazquez, A., Regodon, S., Franco, A., & Redondo, E. (1995): Lesions in lambs experimentally infected. Histol Histopathol, 10, 71-77. http://hdl.handle.net/10201/18701
Masot, A., Kelling, C. L., Lopez, O., Sur, J., & Redondo, E. (2000): In situ hybridization detection of bovine respiratory syncytial virus in the lung of experimentally infected lambs. Vet Pathol, 37(6), 618-625. https://doi.org/https://doi.org/10.1354/vp.37-6-618
Narita, M., Yamada, M., Tsuboi, T., & Kawashima, K. (2002): Immunohistopathology of calf pneumonia induced by endobronchial inoculation with bovine adenovirus 3. Vet Pathol, 39(5), 565-571. https://doi.org/https://doi.org/10.1354/vp.39-5-565
Newcomer, B. W., Neill, J. D., Galik, P. K., Riddell, K. P., Zhang, Y., Passler, T., Velayudhan, B. T., & Walz, P. H. (2017): Serologic survey for antibodies against three genotypes of bovine parainfluenza 3 virus in unvaccinated ungulates in Alabama. AJVR, 78(2), 239-243. https://doi.org/https://doi.org/10.2460/ajvr.78.2.239
Nicod, L. P. (2005): Lung defences: an overview. Eur Respir Rev, 14(95), 45-50. https://doi.org/https://doi.org/10.1183/09059180.05.00009501
Redondo, E., Gomez, L., Kelling, C., Gazquez, A., & Masot, A. (2003): Bovine respiratory syncytial virus in-situ hybridization from sheep lungs at different times postinfection. Archivos de Medicina Veterinaria, 35(1), 37-49.
Sacco, R. E., McGill, J., Pillatzki, A. E., Palmer, M., & Ackermann, M. R. (2014): Respiratory syncytial virus infection in cattle. Veterinary pathology, 51(2), 427-436. https://doi.org/https://doi.org/10.1177/0300985813501341
Saeed, I. K., Ali, Y. H., Taha, K. M., Mohammed, N. E., Nouri, Y. M., Mohammed, B. A., Mohammed, O. I., Elmagboul, S. B., & AlGhazali, F. A. (2016): Para influenza virus 3 infection in cattle and small ruminants in Sudan. J Adv Vet Anim Res, 3(3), 236-241. https://doi.org/http://doi.org/10.5455/javar.2016.c160
Sharma, R. N., Tiwari, K., Williams, S., Dick-Valdez, M., Tang-Wing, C., & DeAllie, C. (2017): Detection of Antibodies against Bovine Respiratory Syncytial Virus in Ruminants of Grenada, West Indies. Journal of Animal Research, 7(2), 223-226. https://doi.org/https://doi.org/10.5958/2277-940X.2017.00033.X
Sharp, J., Gilmour, N., Thompson, D., & Rushton, B. (1978): Experimental infection of specific pathogen-free lambs with parainfluenza virus type 3 and Pasteurella haemolytica. J COMP PATH, 88(2), 237-243. https://doi.org/https://doi.org/10.1016/0021-9975(78)90027-0
Stevenson, R., & Hore, D. (1970): Comparative pathology of lambs and calves infected with parainfluenza virus type 3. J COMP PATH, 80(4), 613-618. https://doi.org/https://doi.org/10.1016/0021-9975(70)90060-5
Stott, E., & Taylor, G. (1985): Respiratory syncytial virus. Archives of virology, 84(1-2), 1-52. https://doi.org/https://doi.org/10.1007/BF01310552
Tamer, C., Palanci, H. S., Yazici, Z., Bayram, E., Cakmaker, M., Ozan, E., Kadi, H., Onkol, S., Gumusova, S., & Albayrak, H. (2018): Serological data of bovine herpes virus type-1 and bovine viral diarrhea virus infections in various ruminants in small-scale farms in the Central and Eastern Black Sea Region, Turkey. Indian J Anim Res, 52(6), 903-906. https://doi.org/https://doi.org/10.18805/ijar.v0iOF.8470
Thomas, L., & Stott, E. (1981): Diagnosis of respiratory syncytial virus infection in the bovine respiratory tract by immunofluorescence. VetRecord, 108(20), 432-435. https://doi.org/https://doi.org/10.1136/vr.108.20.432
Tiwari, K., Cornish, C., Gamble, B., Thomas, D., & Sharma, R. N. (2016): Seroprevalence of bovine parainfluenza virus Type 3 (bPI-3V) in ruminants from Grenada. Open Journal of Veterinary Medicine, 6(2), 23-27. https://doi.org/https://doi.org/10.4236/ojvm.2016.62004
Van der Poel, W., Brand, A., Kramps, J., & Van Oirschot, J. (1994): Respiratory syncytial virus infections in human beings and in cattle. Journal of Infection, 29(2), 215-228. https://doi.org/https://doi.org/10.1016/S0163-4453(94)90866-4 Van der Poel, W., Langedijk, J., Kramps, J., Middel, W., Brand, A., & Van Oirschot, J. (1995): Bovine respiratory syncytial virus antibodies in non-bovine species. Arch Virol, 140(9), 1549-1555. https://doi.org/https://doi.org/10.1007/BF01322529
Viuff, B., Uttenthal, Å., Tegtmeier, C., & Alexandersen, S. (1996): Sites of replication of bovine respiratory syncytial virus in naturally infected calves as determined by in situ hybridization. Vet Pathol, 33(4), 383-390. https://doi.org/https://doi.org/10.1177/030098589603300403
Yavuz, O., & Dinçel, G. Ç. (2020): Expression of COX-2, HMGB-1 and CD68 in lung tissue in sheep fibrinous bronchopneumonia. Med. Weter, 76(01), 46-52. https://doi.org/https://doi.org/10.21521/mw.6341
Yener, Z., Sağlam, Y., Timurkaan, N., & Ilhan, F. (2005): Immunohistochemical detection of parainfluenza type 3 virus antigens in paraffin sections of pneumonic caprine lungs. J Vet Med, 52(6), 268-271. https://doi.org/https://doi.org/10.1111/j.1439-0442.2005.00724.x
Yeşilbağ, K., & Güngör, B. (2009): Antibody prevalence against respiratory viruses in sheep and goats in North-Western Turkey. Trop Anim Health Prod, 41(4), 421-425. https://doi.org/https://doi.org/10.1007/s11250-008-9225-3
Zaher, K. S., Syame, S. M., Elhewairy, H. M., & Marie, H. S. (2014): Investigation of bovine respiratory disease complex in Egypt with emphasis on some viral and bacterial pathogens. Life Sci J, 11(6), 56-62. https://doi.org/http://www.lifesciencesite.com.