Çeşitli kaynaklardan izole edilen bazı mikroorganizmaların sistein üretim kapasitelerinin belirlenmesi

Bu çalışmada, çeşitli kaynaklardan (toprak ve çeşitli peynirler) izole edilen mikroorganizmaların sistein üretme kapasitelerinin belirlenmesi hedeflenmiştir. Bu amaçla toplamda 9 farklı kaynaktan 262 bakteri izole edildi; bunların ürediği besiyeri ortamlarının hepsinde değişen oranlarda sistein varlığı tespit edildi. Bakterilerin sistein üretme kapasitesi (0.452-1.855 g/L aralığında), Nurtient Broth kullanılarak 37 °C’de 72 saat fermantasyon işlemi sonucunda spektrofotometrik yöntemle belirlendi. Sistein, serin ve metionin amino asitlerinin FTIR karakterizasyonu yapıldı ve ayrıca değişen konsantrasyonlarda sistein içeren sulu çözeltilerinin FTIR spektrumları incelendi. Elde edilen spektrumlara göre, sistein konsantrasyonuna bağlı olarak band yoğunlukların farklılaştığı sonucuna varıldı.

Anahtar Kelimeler:

Amino asit üreten bakteriler, mikrobiyal sistein üretimi, ticari sistein üretimi

Determination of cysteine production capacity of some microorganisms isolated from various sources

In this study, it was aimed to determine the cysteine production capacity of microorganisms isolated from various sources (soil and various types of cheese). For this purpose, 262 bacteria were isolated from 9 different sources. Cysteine was present in varied concentrations (0.452-1.855 g/L) in all of the liquid media. The cysteine concentration was determined by spectrophotometric method after fermentation process using Nurtient Broth at 37 °C for 72 hours. FTIR characterization of the cysteine, serine and methionine amino acids was performed, and also the FTIR spectra of aqueous solutions containing cysteine at varying concentrations were examined to evaluate the differences in spectrums. According to the spectrums obtained, it was concluded that the peak intensities were changed based on the concentration of cysteine.

Keywords:

Amino acid producing bacteria, microbial production of sistein, commercial production of sistein,

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İstanbul Sabahattin Zaim Üniversitesi Fen Bilimleri Enstitüsü Dergisi-Cover

Yayın Aralığı: Yılda 2 Sayı
Başlangıç: 2019
Yayıncı: İstanbul Sabahattin Zaim Üniversitesi

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