THE EVALUATION OF TURKISH AUTUMN FLOWERING COLCHICUM SPECIES

65 Colchicum species grow all over the World and 25 of them grow inTurkey.In this work, Turkish Colchicum species with respect to the tropolone alkaloidsthat have gained importance in pharmaceutical industry have been evaluated.First of all, the preparative TLC separation and spectrophotometric determinationof colchicine and demecolcine were accomplished. Species with lowalkaloid content and with small seeds and corms which have not economic valuewere eliminated and 10 Colchicum species were investigated with respect totheir total tropolonic alkaloid content using a direct spectrophotometric method.HPLC was also used to quantitate the amount of colchicine, colchicoside anddemecolcine. The results obtained were compared with those of C.autumnalecited in the Pharmacopeiae.As a result of our investigations, alkaloid content of C.baytopiol-urn, bivonae,bol-nmuelleri, cilicicum, kotschyi and speciosum species were found asrich as or even richer than C.autumnale.Key words: Colchicum, Colchicine, Colchicoside, Demecolcine, Tropo-Ionic Alkaloids.

Anahtar Kelimeler:

-

THE EVALUATION OF TURKISH AUTUMN FLOWERING COLCHICUM SPECIES

65 Colchicum species grow all over the World and 25 of them grow in Turkey. In this work, Turkish Colchicum species with respect to the tropolone alkaloids that have gained importance in pharmaceutical industry have been evaluated. First of all, the preparative TLC separation and spectrophotometric determination of colchicine and demecolcine were accomplished. Species with low alkaloid content and with small seeds and corms which have not economic value were eliminated and 10 Colchicum species were investigated with respect to their total tropolonic alkaloid content using a direct spectrophotometric method. HPLC was also used to quantitate the amount of colchicine, colchicoside and demecolcine. The results obtained were compared with those of C.autumnale cited in the Pharmacopeiae. As a result of our investigations, alkaloid content of C.baytopiol-urn, bivonae, bol-nmuelleri, cilicicum, kotschyi and speciosum species were found as rich as or even richer than C.autumnale.

Keywords:

Colchicum, Colchicine, Colchicoside, Demecolcine TropoI lonic Alkaloids,

PDF

___

1. Willis, J.C., A Dictionary of the Flowering Plants & Ferns 265, University Press, Cambridge (1966).
2. Brickell, C.D. in Davis, P.H.: Flora of Turkey & the East Aegean Islands, Vo1.8, p. 329-351, Edinburgh University Press, Edinburgh (1984).
3. Siitliiplnar, N., Tiirkiye'de Yeti~en ve SonbahafPa Ciqek Agan Colchicum Tiirlerinin Kol~isin ve Demekolsin Yoniinden Arqhnlrnasl, TUBITAK, TBAG-399, Istanbul (I98 1).
4. Siitliipmar, N., Tiirkiye'nin Sonbahar Colchicum Tiirlerinin Demekolsin, Kol~ikozit ve Kolaisin Yoniinden Degerlendirilmesi, 1.U. Eczaclllk Fakiiltesi Do~entlik tezi, Istanbul (1985).
5. Siitliipmar, N., Husek, A., PotSsilovi, H., D'vorickovi,~., Hanus, V., Sedmera, P., Siminek, V. Planta Medica, 54,243-245 (1988).
6. Husek, A., Siitliiplnar, N., PotCsilovi, H., DvorBckovB, S., Hanus, V., Sedmera, P., Malon, P., Siminek, V. Phytockemistry, 28,3217-3219 (1989).
7. Husek, A., Siitliipmar, N., ~edhera, P., Siminek, V. Heterocycles, 28,79-82 (1989).
8. Siminek, V., Husek, A., Vilka, I., Siitliipmar, N. Herba Hungarica, 29,64-68 (1990)